Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TrkB antibody (GTX133722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with TrkB antibody (GTX133722) diluted at 1:1000 and competitor's antibody (sc-12) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TrkB antibody detects TrkB protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TrkB protein stained by TrkB antibody (GTX133722) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

TrkB antibody detects TrkB protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TrkB stained by TrkB antibody (GTX133722) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TrkB antibody detects TrkB protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TrkB stained by TrkB antibody (GTX133722) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min