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TrkB antibody

Comparison
Anti-TrkB antibody used in Western Blot (WB). GTX133722
Anti-TrkB antibody used in IHC (Paraffin sections) (IHC-P). GTX133722
Anti-TrkB antibody used in IHC (Paraffin sections) (IHC-P). GTX133722
Anti-TrkB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133722
Anti-TrkB antibody used in Western Blot (WB). GTX133722

Cat. No. GTX133722

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TrkB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Positive Control

mouse brain

Predict Reactivity

Human, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of mouse TrkB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887078

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurotrophic tyrosine kinase, receptor, type 2 , GP145-TrkB/GP95-TrkB , Tkrb , trk-B , trkB

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Database

Research Area

DATA IMAGES

Anti-TrkB antibody used in Western Blot (WB). GTX133722

GTX133722 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TrkB antibody (GTX133722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TrkB antibody used in IHC (Paraffin sections) (IHC-P). GTX133722

GTX133722 IHC-P Image

TrkB antibody detects TrkB protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TrkB stained by TrkB antibody (GTX133722) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TrkB antibody used in IHC (Paraffin sections) (IHC-P). GTX133722

GTX133722 IHC-P Image

TrkB antibody detects TrkB protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TrkB stained by TrkB antibody (GTX133722) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TrkB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133722

GTX133722 ICC/IF Image

TrkB antibody detects TrkB protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TrkB protein stained by TrkB antibody (GTX133722) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-TrkB antibody used in Western Blot (WB). GTX133722

GTX133722 WB Image

Comparison

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with TrkB antibody (GTX133722) diluted at 1:1000 and competitor's antibody (sc-12) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

Application Reference

Stefania Zorzin et al. Int J Mol Sci 2021; 22 (19) Environmental Enrichment Induces Meningeal Niche Remodeling through TrkB-Mediated Signaling.
Application : WB
Reactivity : Mouse
Chang JW et al. Cereb Cortex 2020; 30 (8) : 4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
Application : WB
Reactivity : Rat
Liang M et al. Neuroscience 2019; 422 : 21-31 Postnatal Lipopolysaccharide Exposure Impairs Adult Neurogenesis and Causes Depression-like Behaviors Through Astrocytes Activation Triggering GABAA Receptor Downregulation.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169