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TrkB antibody

Anti-TrkB antibody used in Western Blot (WB). GTX54857
Anti-TrkB antibody used in Flow cytometry (FACS). GTX54857
Anti-TrkB antibody used in Western Blot (WB). GTX54857
Anti-TrkB antibody used in IHC (Frozen sections) (IHC-Fr). GTX54857
Anti-TrkB antibody used in Live cell imaging (LCI). GTX54857

Cat. No. GTX54857

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, FACS, LCI

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all TrkB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)RLEPNSIDPENITE, corresponding to amino acid residues 57-70 (Extracellular domain) of rat TrkB (Accession  : Q63604).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887896

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurotrophic receptor tyrosine kinase 2 , RATTRKB1 , TRKB1 , Tkrb , trk-B , trkB

Cellular Localization

Cell membrane,Endosome membrane,Cell projection, dendrite

Background

receptor for brain-derived neurotrophic factor (BDNF) [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-TrkB antibody used in Western Blot (WB). GTX54857

GTX54857 WB Image

WB analysis of rat brain membranes (lanes 1 and 3) and HEK-TrkB transfected (lanes 2 and 4) lysates using GTX54857 TrkB antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TrkB antibody used in Flow cytometry (FACS). GTX54857

GTX54857 FACS Image

FACS analysis of HL-60 cells using GTX54857 TrkB antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Dilution : 1:25

Anti-TrkB antibody used in Western Blot (WB). GTX54857

GTX54857 WB Image

WB analysis of mouse brain tissue lysate using GTX54857 TrkB antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TrkB antibody used in IHC (Frozen sections) (IHC-Fr). GTX54857

GTX54857 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX54857 TrkB antibody. TrkB (red) is expressed in DRG neurons. Hoechst 33342 is used as the counterstain.
Dilution : 1:100

Anti-TrkB antibody used in Live cell imaging (LCI). GTX54857

GTX54857 LCI Image

Live cell imaging analysis of live intact PC12 cells using GTX54857 TrkB antibody.
Panel A : Primary antibody (Red)
Panel B : Live view of the cell
Panel C : Merged images of Panel A and B
Dilution : 1:50

REFERENCE

Application Reference

Kumro J et al. Cereb Cortex 2023; 33 (12) : 7627-7641 Chronic basal forebrain activation improves spatial memory, boosts neurotrophin receptor expression, and lowers BACE1 and Aβ42 levels in the cerebral cortex in mice.
Kim HI et al. Neurotherapeutics 2022; 19 (4) : 1298-1312 ERRγ Ligand Regulates Adult Neurogenesis and Depression-like Behavior in a LRRK2-G2019S-associated Young Female Mouse Model of Parkinson's Disease.
Lih-Geeng Chen et al. Biomedicines 2022; 10 (2) Vitisin A, a Resveratrol Tetramer, Improves Scopolamine-Induced Impaired Learning and Memory Functions in Amnesiac ICR Mice.
Application : WB
Reactivity : Mouse
Fang Yan et al. BMC Cardiovasc Disord 2022; 22 (1) : 182 H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure.
Application : WB
Reactivity : Rat
CH Chang et al. Mol Ther Nucleic Acids 2020; 20 : 812–822 MicroRNA-206 Regulates Stress-Provoked Aggressive Behaviors in Post-weaning Social Isolation Mice
Application : WB
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REVIEW

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Application Reference
Kumro J et al. Cereb Cortex 2023; 33 (12):7627-7641 Chronic basal forebrain activation improves spatial memory, boosts neurotrophin receptor expression, and lowers BACE1 and Aβ42 levels in the cerebral cortex in mice.
Kim HI et al. Neurotherapeutics 2022; 19 (4):1298-1312 ERRγ Ligand Regulates Adult Neurogenesis and Depression-like Behavior in a LRRK2-G2019S-associated Young Female Mouse Model of Parkinson's Disease.
Lih-Geeng Chen et al. Biomedicines 2022; 10 (2) Vitisin A, a Resveratrol Tetramer, Improves Scopolamine-Induced Impaired Learning and Memory Functions in Amnesiac ICR Mice.
Application : WB
Reactivity : Mouse
Fang Yan et al. BMC Cardiovasc Disord 2022; 22 (1):182 H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure.
Application : WB
Reactivity : Rat
CH Chang et al. Mol Ther Nucleic Acids 2020; 20:812–822 MicroRNA-206 Regulates Stress-Provoked Aggressive Behaviors in Post-weaning Social Isolation Mice
Application : WB
Zhang H et al. J Comp Neurol 2018; 526 (15):2482-2492 Ablation of ErbB4 in parvalbumin-positive interneurons inhibits adult hippocampal neurogenesis through down-regulating BDNF/TrkB expression.
Application : WB
Reactivity : Mouse
Botsakis K et al. Neuropharmacology 2017; 121:140-157 BNN-20, a synthetic microneurotrophin, strongly protects dopaminergic neurons in the "weaver" mouse, a genetic model of dopamine-denervation, acting through the TrkB neurotrophin receptor.
Application : IHC-Fr
Reactivity : Mouse
Chang CY et al. Biomed Res Int 2015; 2015:159015 Subchronic Arsenic Exposure Induces Anxiety-Like Behaviors in Normal Mice and Enhances Depression-Like Behaviors in the Chemically Induced Mouse Model of Depression.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639