APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Chicken, Pig, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Trk B around the phosphorylation site of Tyr516.
Purification
Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
GP145TrkB , NTRK2 , TRKB , neurotrophic receptor tyrosine kinase 2 , trkB , TrkB , Trk B
Cellular Localization
Cell membrane,Endosome membrane,Early endosome membrane
Background
This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Database
Research Area
DATA IMAGES
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GTX00804 IHC-P Image
IHC-P analysis of staining human brain tissue using GTX00804 TrkB (phospho Tyr515) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX00804 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00804 TrkB (phospho Tyr515) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX00804 WB Image
WB analysis of extracts from rat liver and mouse brain tissue lysate using GTX00804 TrkB (phospho Tyr515) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
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GTX00804 ICC/IF Image
ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00804 TrkB (phospho Tyr515) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200
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REFERENCE
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REVIEW
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