ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00804 TrkB (phospho Tyr515) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

WB analysis of extracts from rat liver and mouse brain tissue lysate using GTX00804 TrkB (phospho Tyr515) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00804 TrkB (phospho Tyr515) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

IHC-P analysis of staining human brain tissue using GTX00804 TrkB (phospho Tyr515) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100