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TrkB (phospho Tyr516) antibody

Anti-TrkB (phospho Tyr516) antibody used in IHC (Paraffin sections) (IHC-P). GTX00804
Anti-TrkB (phospho Tyr516) antibody used in Western Blot (WB). GTX00804
Anti-TrkB (phospho Tyr516) antibody used in ELISA (ELISA). GTX00804
Anti-TrkB (phospho Tyr516) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00804

Cat No. GTX00804

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

92 kDa. ( Note )

Predict Reactivity

Rabbit, Sheep, Chicken, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Trk B around the phosphorylation site of Tyr516.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GP145TrkB,NTRK2,TRKB,neurotrophic receptor tyrosine kinase 2,trkB,TrkB,Trk B

Cellular Localization

Cell membrane,Endosome membrane,Early endosome membrane

Background

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-TrkB (phospho Tyr516) antibody used in IHC (Paraffin sections) (IHC-P). GTX00804

GTX00804 IHC-P Image

IHC-P analysis of staining human brain tissue using GTX00804 TrkB (phospho Tyr516) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-TrkB (phospho Tyr516) antibody used in Western Blot (WB). GTX00804

GTX00804 WB Image

WB analysis of extracts from rat liver and mouse brain tissue lysate using GTX00804 TrkB (phospho Tyr516) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-TrkB (phospho Tyr516) antibody used in ELISA (ELISA). GTX00804

GTX00804 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00804 TrkB (phospho Tyr516) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-TrkB (phospho Tyr516) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00804

GTX00804 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00804 TrkB (phospho Tyr516) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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