GeneTex
United States (US)

TrkC antibody

Anti-TrkC antibody used in Western Blot (WB). GTX107385
Anti-TrkC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107385

Cat No. GTX107385

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TrkC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neurotrophic Receptor Tyrosine Kinase 3,Gp145-Trkc,Trkc,Gp145(Trkc),Ntrk3

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-TrkC antibody used in Western Blot (WB). GTX107385

GTX107385 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TrkC antibody (GTX107385) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TrkC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107385

GTX107385 ICC/IF Image

TrkC antibody detects TrkC protein at cytoplasm by immunofluorescent analysis.
Sample: SKNSH cells were fixed in ice-cold MeOH for 5 min.
Green: TrkC protein stained by TrkC antibody (GTX107385) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf