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Trkct1 antibody

Anti-Trkct1 antibody used in Western Blot (WB). GTX48720
Anti-Trkct1 antibody used in Immunoprecipitation (IP). GTX48720
Anti-Trkct1 antibody used in Western Blot (WB). GTX48720

Cat No. GTX48720

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, ELISA

Reactivity

Human, Mouse, Rat
Package
50 μg ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:10000
IP 1 μg
ELISA 1:5000 - 1:20000
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse TrkCT1 protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

neurotrophic tyrosine kinase, receptor, type 3 , AW125844 , Ntrk3_tv3 , TrkC

Cellular Localization

Membrane

Background

TrkCT1, also named neurotrophic tyrosine kinase receptor type 3 (Ntrk3) non-catalytic isoform 2, is a non-catalytic isoform of TrkC, the high affinity receptor for Neurotrophin-3 (NT-3). This isoform lacks the kinase domain that is responsible for signaling by the full-length isoform. TrkCT1 is the product of an alternative splicing of Ntrk3 that leaves the extracellular and transmembrane domains intact but includes a shorter intracellular domain encoded by exons 13b and 14b. Recent studies indicate that the short cytoplasmic tail binds the scaffold protein Tamalin in a ligand-dependent manner and further activates the Arf6–Rac1 signaling pathway (Esteban et al., 2006).

Database

Research Area

DATA IMAGES

Anti-Trkct1 antibody used in Western Blot (WB). GTX48720

GTX48720 WB Image

Western blot using GeneTex's affinity purified anti-TrkCT1 to detect endogenous TrkCT1 in mouse cortex lysate (Lane 1). Lane 2 is TrkCT1 knock-out cortex lysate. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:6,000 dilution.

Anti-Trkct1 antibody used in Immunoprecipitation (IP). GTX48720

GTX48720 IP Image

Mouse cortex lysate was immunoprecipitated with anti-TrkCT1 antibody and further blotted with affinity purified anti-TrkCT1. Lane 1 is wild-type cortex lysate, Lane 2 is Tamalin knock-out cortex lysate, and Lane 3 is TrkCT1 knock-out cortex lysate. The membrane was probed with the primary antibody at a 1:6,000 dilution.

Anti-Trkct1 antibody used in Western Blot (WB). GTX48720

GTX48720 WB Image

Western blot using GeneTex's affinity purified anti-TrkCT1 to detect over-expressed TrkCT1 in HEK293 cells (Lane 2, arrowhead). Lane 1 is a non-transfected control. Cell extracts were resolved by electrophoresis and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:3,000 dilution.

REFERENCE

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SDS
Sodium Azide.pdf