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Tropomodulin 2 antibody [N3C3]

Anti-Tropomodulin 2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119685
Anti-Tropomodulin 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX119685
Anti-Tropomodulin 2 antibody [N3C3] used in Western Blot (WB). GTX119685
Anti-Tropomodulin 2 antibody [N3C3] used in Western Blot (WB). GTX119685

Cat. No. GTX119685

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Tropomodulin 2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

MCF-7 , Mouse brain

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tropomodulin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617439

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tropomodulin 2 , N-TMOD , NTMOD

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a neuronal-specific member of the tropomodulin family of actin-regulatory proteins. The encoded protein caps the pointed end of actin filaments preventing both elongation and depolymerization. The capping activity of this protein is dependent on its association with tropomyosin. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tropomodulin 2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119685

GTX119685 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using Tropomodulin 2(GTX119685) antibody at 1:200 dilution.

Anti-Tropomodulin 2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX119685

GTX119685 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using Tropomodulin 2(GTX119685) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tropomodulin 2 antibody [N3C3] used in Western Blot (WB). GTX119685

GTX119685 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX119685 diluted at 1:1000

Anti-Tropomodulin 2 antibody [N3C3] used in Western Blot (WB). GTX119685

GTX119685 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
10% SDS PAGE
GTX119685 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169