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Tropomyosin 1 antibody

Anti-Tropomyosin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113857
Anti-Tropomyosin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113857
Anti-Tropomyosin 1 antibody used in Western Blot (WB). GTX113857
Anti-Tropomyosin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113857
Anti-Tropomyosin 1 antibody used in Western Blot (WB). GTX113857
Anti-Tropomyosin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113857
Anti-Tropomyosin 1 antibody used in Western Blot (WB). GTX113857
Anti-Tropomyosin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113857

Cat. No. GTX113857

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μl ($419),
25 μl ($169)
See all Tropomyosin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Mouse liver , rat muscle

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tropomyosin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038154

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tropomyosin 1 , C15orf13 , CMD1Y , CMH3 , HEL-S-265 , HTM-alpha , LVNC9 , TMSA

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tropomyosin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113857

GTX113857 IHC-P Image

Tropomyosin 1 antibody detects Tropomyosin 1 protein at cytosol on mouse vein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse vein.
Tropomyosin 1 antibody (GTX113857) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tropomyosin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113857

GTX113857 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Tropomyosin 1(GTX113857) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tropomyosin 1 antibody used in Western Blot (WB). GTX113857

GTX113857 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tropomyosin 1 antibody (GTX113857) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tropomyosin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX113857

GTX113857 IHC-P Image

Tropomyosin 1 antibody detects Tropomyosin 1 protein at cytosol on mouse vein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse vein.
Tropomyosin 1 antibody (GTX113857) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tropomyosin 1 antibody used in Western Blot (WB). GTX113857

GTX113857 WB Image

Tropomyosin 1 antibody detects TPM1 protein by western blot analysis.
A. 50 μg rat muscle lysate/extract
10% SDS-PAGE
Tropomyosin 1 antibody (GTX113857) dilution: 1:6000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tropomyosin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113857

GTX113857 ICC/IF Image

Tropomyosin 1 antibody detects Tropomyosin 1 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tropomyosin 1 stained by Tropomyosin 1 antibody (GTX113857) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Tropomyosin 1 antibody used in Western Blot (WB). GTX113857

GTX113857 WB Image

Sample (50 μg of whole cell lysate)
A: mouse liver
12% SDS PAGE
GTX113857 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tropomyosin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113857

GTX113857 ICC/IF Image

The data was published in the journal Stem Cells Dev in 2019. PMID: 30803370

REFERENCE

Application Reference

Oldershaw R et al. Stem Cells Dev 2019; 28 (9) : 593-607 Human Cardiac-Mesenchymal Stem Cell-Like Cells, a Novel Cell Population with Therapeutic Potential.
Jie Li et al. Experimental Cell Research 2015; : 1-39 Cytoskeletal Binding Proteins Distinguish Cultured Dental Follicle Cells and Periodontal Ligament Cells.
Application : WB
Reactivity : Human
Huang HJ et al. Mol Biosyst 2014; (Epub) Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Application : WB
Reactivity : Human
Yun-Hong Y et al. Mol Cell Proteomics 2011; 10 (10) : M110.007138 A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum.
Application : WB
Reactivity : Pig
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169