Wild-type (WT) and Tropomyosin 3 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Tropomyosin 3 antibody [N2C3] (GTX113568) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Tropomyosin 3 antibody [N2C3] detects Tropomyosin 3 protein at cytosol on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Tropomyosin 3 antibody [N2C3] (GTX113568) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min