GeneTex
United States (US)

Tropomyosin 4 antibody

GTX114851_40233_WB_M_brain_18121410_437.jpg
GTX114851_WB_18121410_776.jpg
GTX114851_40233_IHC_18121410_173.jpg

Cat No. GTX114851

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

NT2D1 , HeLa , mouse brain

Predict Reactivity

Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tropomyosin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tropomyosin 4,Hel-S-108,Tpm4

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

DATA IMAGES
GTX114851_40233_WB_M_brain_18121410_437.jpg

GTX114851 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114851 diluted at 1:1000

GTX114851_WB_18121410_776.jpg

GTX114851 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: Hela
12% SDS PAGE
GTX114851 diluted at 1:1000

GTX114851_40233_IHC_18121410_173.jpg

GTX114851 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using Tropomyosin 4(GTX114851) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169