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Tropomyosin 4 antibody

Orthogonal
Anti-Tropomyosin 4 antibody used in IHC (Whole mount) (IHC-Wm). GTX114851
Anti-Tropomyosin 4 antibody used in Western Blot (WB). GTX114851
Anti-Tropomyosin 4 antibody used in Western Blot (WB). GTX114851
Anti-Tropomyosin 4 antibody used in Western Blot (WB). GTX114851
Anti-Tropomyosin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114851
Anti-Tropomyosin 4 antibody used in Western Blot (WB). GTX114851

Cat. No. GTX114851

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Tropomyosin 4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Mouse brain , HeLa , NT2D1

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tropomyosin 4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620707

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tropomyosin 4 , HEL-S-108

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tropomyosin 4 antibody used in IHC (Whole mount) (IHC-Wm). GTX114851

GTX114851 IHC-Wm Image

Tropomyosin 4 antibody detects Tropomyosin 4 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tropomyosin 4 antibody (GTX114851) dilution: 1:100.
Anti-Tropomyosin 4 antibody used in Western Blot (WB). GTX114851

GTX114851 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish heart
B: zebrafish muscle
12% SDS PAGE
GTX114851 diluted at 1:500

Anti-Tropomyosin 4 antibody used in Western Blot (WB). GTX114851

GTX114851 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114851 diluted at 1:1000

Anti-Tropomyosin 4 antibody used in Western Blot (WB). GTX114851

GTX114851 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: Hela
12% SDS PAGE
GTX114851 diluted at 1:1000

Anti-Tropomyosin 4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114851

GTX114851 IHC-P Image

Immunohistochemical analysis of paraffin-embedded 4T1 xenograft, using Tropomyosin 4(GTX114851) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tropomyosin 4 antibody used in Western Blot (WB). GTX114851

GTX114851 WB Image

Orthogonal

The data was published in the journal J Biomed Sci in 2013. PMID: 24359494

REFERENCE

Application Reference

Levine J et al. Glia 2015; Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival.
Application : WB
Reactivity : Mouse
Chen JY et al. J Biomed Sci 2013; 20 : 95 quercetin-induced cardioprotection against * cytotoxicity.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169