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Tropomyosin antibody [SPM224]

Anti-Tropomyosin antibody [SPM224] used in IHC (Paraffin sections) (IHC-P). GTX17784

Cat. No. GTX17784

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM224

Isotype

 IgG1

Application

 ICC/IF, IHC-P

Reactivity

 Human, Rat, Chicken
Package
500 μl ($379)
See all Tropomyosin products

APPLICATION

Application Note

For IF: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Skeletal or cardiac muscle

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.4, 0.6% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

Full length native protein (purified) (Chicken).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TPM3 , TPM1 , BRT2 , BRT-2 , 396366 , Tropomyosin , 396430 , BRT 2

Cellular Localization

Cytoplasmic

Background

Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.

Research Area

DATA IMAGES

Anti-Tropomyosin antibody [SPM224] used in IHC (Paraffin sections) (IHC-P). GTX17784

GTX17784 IHC-P Image

Human heart stained with GTX17784 at 1/50 on paraffin sections

REFERENCE

Application Reference

Furukawa N et al. Heart Vessels 2021; 36 (1) : 136-146 DPP-4 inhibitor induces FGF21 expression via sirtuin 1 signaling and improves myocardial energy metabolism.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 379