GeneTex
  • Country / Location Selection

United States (US)

Troponin T fast skeletal antibody

Anti-Troponin T fast skeletal antibody used in Western Blot (WB). GTX130922
Anti-Troponin T fast skeletal antibody used in Western Blot (WB). GTX130922
Anti-Troponin T fast skeletal antibody used in Western Blot (WB). GTX130922

Cat No. GTX130922

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

human muscle , mouse muscle , rat muscle

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Troponin T fast skeletal. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

troponin T3, fast skeletal type,TNTF,beta-TnTF

Background

The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Troponin T fast skeletal antibody used in Western Blot (WB). GTX130922

GTX130922 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNNT3 antibody (GTX130922) diluted at 1:5000.

Anti-Troponin T fast skeletal antibody used in Western Blot (WB). GTX130922

GTX130922 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNNT3 antibody (GTX130922) diluted at 1:5000.

Anti-Troponin T fast skeletal antibody used in Western Blot (WB). GTX130922

GTX130922 WB Image

Human tissue extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNNT3 antibody (GTX130922) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 21490260

REFERENCE
There are currently no references for Troponin T fast skeletal antibody (GTX130922). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Troponin T fast skeletal antibody (GTX130922). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169