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Troponin T1 (slow) skeletal muscle antibody

Anti-Troponin T1 (slow) skeletal muscle antibody used in IHC (Paraffin sections) (IHC-P). GTX109585
Anti-Troponin T1 (slow) skeletal muscle antibody used in Western Blot (WB). GTX109585
Anti-Troponin T1 (slow) skeletal muscle antibody used in IHC (Paraffin sections) (IHC-P). GTX109585

Cat No. GTX109585

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Pig
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

H1299 , *Porcine skeletal muscle

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Troponin T1 (slow) skeletal muscle Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952333

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

troponin T1, slow skeletal type , ANM , NEM5 , STNT , TNT , TNTS

Background

This gene encodes a protein that is a subunit of troponin, which is a regulatory complex located on the thin filament of the sarcomere. This complex regulates striated muscle contraction in response to fluctuations in intracellular calcium concentration. This complex is composed of three subunits: troponin C, which binds calcium, troponin T, which binds tropomyosin, and troponin I, which is an inhibitory subunit. This protein is the slow skeletal troponin T subunit. Mutations in this gene cause nemaline myopathy type 5, also known as Amish nemaline myopathy, a neuromuscular disorder characterized by muscle weakness and rod-shaped, or nemaline, inclusions in skeletal muscle fibers which affects infants, resulting in death due to respiratory insufficiency, usually in the second year. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Troponin T1 (slow) skeletal muscle antibody used in IHC (Paraffin sections) (IHC-P). GTX109585

GTX109585 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Troponin T1 (slow skeletal)(GTX109585) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Troponin T1 (slow) skeletal muscle antibody used in Western Blot (WB). GTX109585

GTX109585 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
GTX109585 diluted at 1:1000

Anti-Troponin T1 (slow) skeletal muscle antibody used in IHC (Paraffin sections) (IHC-P). GTX109585

GTX109585 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Porcine skeletal muscle, using Troponin T1(GTX109585) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for Troponin T1 (slow) skeletal muscle antibody (GTX109585). Be the first to share your publications with this product.

REVIEW

Troponin T1 (slow) skeletal muscle antibody Cat No. GTX109585

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 27-Jun-2012
Score :
Application Tested : IHC-P
Sample Species : Pig
Sample : skeletal muscle tissue
Blocking : 3% H2O2 , 25°C, 0.5Hr
Fixation : 10% neutral buffered formalin
Permeabilization /Antigen retrieval : Citrate
Primary Antibody : 1:500, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169