APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Observed MW
55 kDa. The observed M.W. is based on the following publication. PMID: 30677045
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT30F1 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TXNRD3 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thioredoxin reductase 1 , GRIM12 , TR , TR1 , TRXR1 , TXNR , TXNRD1 , TrxR1 , Thioredoxin Reductase 1
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis. [provided by RefSeq, May 2017]
Database
Research Area
DATA IMAGES
![Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697](/templates/default/js/lightbox/images/loading.gif "Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697")
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GTX57697 ICC/IF Image
ICC/IF analysis of A549 cells using GTX57697 TrxR1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57697 WB Image
WB analysis of various samples using GTX57697 TrxR1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : PC-3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57697 ICC/IF Image
ICC/IF analysis of Hep3B cells using GTX57697 TrxR1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
|
REFERENCE
There are currently no references for TrxR1 antibody [AT30F1] (GTX57697). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TrxR1 antibody [AT30F1] (GTX57697). Be the first to share your experience with this product.
![Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697](/upload/website/prouct_img/small/GTX57697/GTX57697_20200204_IF2_w_23061123_716.webp "Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697")
![Anti-TrxR1 antibody [AT30F1] used in Western Blot (WB). GTX57697](/upload/website/prouct_img/small/GTX57697/GTX57697_20200204_WB_w_23061123_799.webp "Anti-TrxR1 antibody [AT30F1] used in Western Blot (WB). GTX57697")
![Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697](/upload/website/prouct_img/small/GTX57697/GTX57697_20200204_IF1_w_23061123_595.webp "Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697")
![Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697](/upload/website/prouct_img/normal/GTX57697/GTX57697_20200204_IF2_w_23061123_716.webp "Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697")
![Anti-TrxR1 antibody [AT30F1] used in Western Blot (WB). GTX57697](/upload/website/prouct_img/normal/GTX57697/GTX57697_20200204_WB_w_23061123_799.webp "Anti-TrxR1 antibody [AT30F1] used in Western Blot (WB). GTX57697")
![Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697](/upload/website/prouct_img/normal/GTX57697/GTX57697_20200204_IF1_w_23061123_595.webp "Anti-TrxR1 antibody [AT30F1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57697")