APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2
Predict Reactivity
Rat, Rabbit, Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human TrxR1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1952464
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
thioredoxin reductase 1 , GRIM-12 , TR , TR1 , TRXR1 , TXNR
Cellular Localization
Cytoplasm , Isoform 4: Cytoplasm , Nucleus , Isoform 5: Cytoplasm
Background
This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202](/templates/default/js/lightbox/images/loading.gif "Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202")
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GTX103202 IHC-P Image
TrxR1 antibody [N1N3] detects TrxR1 protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
TrxR1 antibody [N1N3] (GTX103202) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103202 WB Image
Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX103202 diluted at 1:1000
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GTX103202 IHC-P Image
TrxR1 antibody [N1N3] detects TrxR1 protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
TrxR1 antibody [N1N3] (GTX103202) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103202 IHC-P Image
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using TXNRD1(GTX103202) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103202 ICC/IF Image
TrxR1 antibody [N1N3] detects TrxR1 protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TrxR1 protein stained by TrxR1 antibody [N1N3] (GTX103202) diluted at 1:500.
Blue: Hoechst 33342 staining.
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REFERENCE
There are currently no references for TrxR1 antibody [N1N3] (GTX103202). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TrxR1 antibody [N1N3] (GTX103202). Be the first to share your experience with this product.
![Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202](/upload/website/prouct_img/small/GTX103202/GTX103202_39862_20150116_IHC_M_w_23060119_515.webp "Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in Western Blot (WB). GTX103202](/upload/website/prouct_img/small/GTX103202/GTX103202_WB_w_23060119_168.webp "Anti-TrxR1 antibody [N1N3] used in Western Blot (WB). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202](/upload/website/prouct_img/small/GTX103202/GTX103202_39862_20150116_IHC_M_2_w_23060119_225.webp "Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202](/upload/website/prouct_img/small/GTX103202/GTX103202_IHC_w_23060119_630.webp "Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103202](/upload/website/prouct_img/small/GTX103202/GTX103202_39862_IFA_w_23060119_904.webp "Anti-TrxR1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202](/upload/website/prouct_img/normal/GTX103202/GTX103202_39862_20150116_IHC_M_w_23060119_515.webp "Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in Western Blot (WB). GTX103202](/upload/website/prouct_img/normal/GTX103202/GTX103202_WB_w_23060119_168.webp "Anti-TrxR1 antibody [N1N3] used in Western Blot (WB). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202](/upload/website/prouct_img/normal/GTX103202/GTX103202_39862_20150116_IHC_M_2_w_23060119_225.webp "Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202](/upload/website/prouct_img/normal/GTX103202/GTX103202_IHC_w_23060119_630.webp "Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202")
![Anti-TrxR1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103202](/upload/website/prouct_img/normal/GTX103202/GTX103202_39862_IFA_w_23060119_904.webp "Anti-TrxR1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103202")