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TrxR1 antibody [N1N3]

Anti-TrxR1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103202
Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202
Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202
Anti-TrxR1 antibody [N1N3] used in Western Blot (WB). GTX103202
Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202

Cat No. GTX103202

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TrxR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

thioredoxin reductase 1 , GRIM-12 , TR , TR1 , TRXR1 , TXNR

Cellular Localization

Cytoplasm , Isoform 4: Cytoplasm , Nucleus , Isoform 5: Cytoplasm

Background

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-TrxR1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103202

GTX103202 ICC/IF Image

TrxR1 antibody [N1N3] detects TrxR1 protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TrxR1 protein stained by TrxR1 antibody [N1N3] (GTX103202) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202

GTX103202 IHC-P Image

TrxR1 antibody [N1N3] detects TrxR1 protein at cytoplasm on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TrxR1 antibody [N1N3] (GTX103202) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202

GTX103202 IHC-P Image

TrxR1 antibody [N1N3] detects TrxR1 protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
TrxR1 antibody [N1N3] (GTX103202) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TrxR1 antibody [N1N3] used in Western Blot (WB). GTX103202

GTX103202 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX103202 diluted at 1:1000

Anti-TrxR1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX103202

GTX103202 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 xenograft, using TXNRD1(GTX103202) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf