TrxR1 antibody [N3C2], Internal

Cat. No. GTX108727

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all TrxR1 products

View product citations for antibody GTX108727 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

mouse brain , Rat brain , Molt-4

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TrxR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thioredoxin reductase 1 , GRIM-12 , TR , TR1 , TRXR1 , TXNR

Cellular Localization

Cytoplasm , Isoform 4: Cytoplasm , Nucleus , Isoform 5: Cytoplasm

Background

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq]

Database

Gene ID: 7296 TXNRD1
UniProt: Q16881 TXNRD1

Research Area

DATA IMAGES

GTX108727 IHC-P Image

TrxR1 antibody [N3C2], Internal detects TrxR1 protein at cytoplasm and nucleus in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
TrxR1 antibody [N3C2], Internal (GTX108727) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108727 WB Image

TrxR1 antibody [N3C2], Internal detects TXNRD1 protein by western blot analysis.
A. 50 μg Rat brain lysate/extract
7.5% SDS-PAGE
TrxR1 antibody [N3C2], Internal (GTX108727) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108727 WB Image

TrxR1 antibody [N3C2], Internal detects TXNRD1 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
TrxR1 antibody [N3C2], Internal (GTX108727) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108727 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TrxR1 (GTX108727) antibody at 1:200 dilution.

GTX108727 WB Image

Sample (30 μg of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX108727 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108727 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using TrxR1 (GTX108727) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108727 IHC-P Image

TrxR1 antibody [N3C2], Internal detects TrxR1 protein at cytoplasm and nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
TrxR1 antibody [N3C2], Internal (GTX108727) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min