GeneTex
United States (US)

TrxR1 antibody

GTX103207_40772_IHC_19011506_343.jpg
GTX103207_40772_IHC_R_19011506_459.jpg
GTX103207_40772_WB_19011506_944.jpg
GTX103207_40772_IHC_M_19011506_799.jpg
GTX103207_40772_IFA_19011506_172.jpg

Cat No. GTX103207

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TrxR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Thioredoxin Reductase 1,Grim-12,Tr,Tr1,Trxr1,Txnr,Txnrd1

Cellular Localization

Cytoplasm , Isoform 4: Cytoplasm , Nucleus , Isoform 5: Cytoplasm

Background

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103207_40772_IHC_19011506_343.jpg

GTX103207 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using TrxR1(GTX103207) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103207_40772_IHC_R_19011506_459.jpg

GTX103207 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using TrxR1(GTX103207) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103207_40772_WB_19011506_944.jpg

GTX103207 WB Image

Sample (30 µg of whole cell lysate)
A: A549
B: H1299
C: HCT116
7.5% SDS PAGE
GTX103207 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103207_40772_IHC_M_19011506_799.jpg

GTX103207 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using TrxR1(GTX103207) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103207_40772_IFA_19011506_172.jpg

GTX103207 ICC/IF Image

TrxR1 antibody detects TrxR1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TrxR1 protein stained by TrxR1 antibody (GTX103207) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169