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TrxR1 antibody

Orthogonal
Anti-TrxR1 antibody used in Western Blot (WB). GTX110589
Anti-TrxR1 antibody used in Western Blot (WB). GTX110589
Anti-TrxR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110589
Anti-TrxR1 antibody used in Western Blot (WB). GTX110589

Cat. No. GTX110589

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TrxR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

mouse liver , A431

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TrxR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952462

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thioredoxin reductase 1 , GRIM-12 , TR , TR1 , TRXR1 , TXNR

Cellular Localization

Cytoplasm , Isoform 4: Cytoplasm , Nucleus , Isoform 5: Cytoplasm

Background

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TrxR1 antibody used in Western Blot (WB). GTX110589

GTX110589 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX110589 diluted at 1:1000

Anti-TrxR1 antibody used in Western Blot (WB). GTX110589

GTX110589 WB Image

TrxR1 antibody detects TXNRD1 protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
TrxR1 antibody (GTX110589) dilution: 1:1000

Anti-TrxR1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110589

GTX110589 IHC-P Image

TrxR1 antibody detects TXNRD1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
TrxR1 antibody (GTX110589) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TrxR1 antibody used in Western Blot (WB). GTX110589

GTX110589 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 27732960

REFERENCE

Application Reference

Arodin Selenius L et al. Antioxidants (Basel) 2019; 8 (5) The Cell Culture Medium Affects Growth, Phenotype Expression and the Response to Selenium Cytotoxicity in A549 and HepG2 Cells.
Application : WB
Reactivity : Human
Sougat Misra et al. Oncotarget 2016; 7 (46) : 74686-74700 Selenite promotes all-trans retinoic acid-induced maturation of acute promyelocytic leukemia cells.
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169