Tryptophanyl tRNA synthetase antibody [HL2186]

Cat. No. GTX638190

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2186
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX638190 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human Tryptophanyl tRNA synthetase

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tryptophanyl-tRNA synthetase , GAMMA-2 , HMN9 , IFI53 , IFP53

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7453 WARS
UniProt: P23381 WARS

Research Area

DATA IMAGES

GTX638190 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638190 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX638190 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638190 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX638190 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638190 ICC/IF Image

Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX638190 IHC-P Image

Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638190 IHC-P Image

Tryptophanyl tRNA synthetase antibody [HL2186] detects Tryptophanyl tRNA synthetase protein at cell membrane and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse adrenal gland.
Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody [HL2186] (GTX638190) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Tryptophanyl tRNA synthetase antibody [HL2186]

Cat. No. GTX638190

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX638190 WB Image

GTX638190 WB Image

GTX638190 WB Image

GTX638190 WB Image

GTX638190 WB Image

GTX638190 ICC/IF Image

GTX638190 IHC-P Image

GTX638190 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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