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Tryptophanyl tRNA synthetase antibody

KO/KD
Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX110223
Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX110223
Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX110223
Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX110223
Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX110223

Cat. No. GTX110223

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Tryptophanyl tRNA synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Tryptophanyl tRNA synthetase

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2038239

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tryptophanyl-tRNA synthetase , GAMMA-2 , HMN9 , IFI53 , IFP53

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX110223

GTX110223 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody (GTX110223) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX110223

GTX110223 IHC-P Image

Tryptophanyl tRNA synthetase antibody detects Tryptophanyl tRNA synthetase protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Tryptophanyl tRNA synthetase antibody (GTX110223) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX110223

GTX110223 IHC-P Image

Tryptophanyl tRNA synthetase antibody detects Tryptophanyl tRNA synthetase protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Tryptophanyl tRNA synthetase antibody (GTX110223) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX110223

GTX110223 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX110223 diluted at 1:1000

Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX110223

GTX110223 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Tryptophanyl tRNA synthetase(GTX110223) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

A Nakashoji et al. Cancer Res Commun 2024; IDENTIFICATION OF MUC1-C AS A TARGET FOR SUPPRESSING PROGRESSION OF HEAD AND NECK SQUAMOUS CELL CARCINOMAS
Application : WB
Yamashita N et al. Mol Cancer Res 2023; 21 (3) : 274-289 MUC1-C Dictates PBRM1-Mediated Chronic Induction of Interferon Signaling, DNA Damage Resistance, and Immunosuppression in Triple-Negative Breast Cancer.
Masayuki Hagiwara et al. Oncoimmunology 2022; 11 (1) : 2029298 MUC1-C integrates type II interferon and chromatin remodeling pathways in immunosuppression of prostate cancer.
Application : WB
Reactivity : Human
Submit a Reference

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Package List Price ($)
$ 399
$ 169