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Tryptophanyl tRNA synthetase antibody

KO/KD Orthogonal
Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX113861
Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX113861
Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX113861
Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX113861
Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX113861

Cat. No. GTX113861

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Tryptophanyl tRNA synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tryptophanyl tRNA synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170576

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tryptophanyl-tRNA synthetase , GAMMA-2 , HMN9 , IFI53 , IFP53

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX113861

GTX113861 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody (GTX113861) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX113861

GTX113861 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Tryptophanyl tRNA synthetase(GTX113861) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX113861

GTX113861 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Tryptophanyl tRNA synthetase(GTX113861) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX113861

GTX113861 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody (GTX113861) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Tryptophanyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX113861

GTX113861 IHC-P Image

Tryptophanyl tRNA synthetase antibody detects Tryptophanyl tRNA synthetase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Tryptophanyl tRNA synthetase stained by Tryptophanyl tRNA synthetase antibody (GTX113861) diluted at 1:85.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169