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Tryptophanyl tRNA synthetase antibody

Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX32939
Anti-Tryptophanyl tRNA synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32939

Cat No. GTX32939

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:10-1:100
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WARS (NP_776049.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tryptophanyl-tRNA synthetase , GAMMA-2 , HMN9 , IFI53 , IFP53

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Tryptophanyl tRNA synthetase antibody used in Western Blot (WB). GTX32939

GTX32939 WB Image

WB analysis of various sample lysates using GTX32939 Tryptophanyl tRNA synthetase antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-Tryptophanyl tRNA synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32939

GTX32939 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32939 Tryptophanyl tRNA synthetase antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf