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Tubg1 antibody

Anti-Tubg1 antibody used in Immunohistochemistry (IHC). GTX124352
Anti-Tubg1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124352
Anti-Tubg1 antibody used in Western Blot (WB). GTX124352
Anti-Tubg1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124352

Cat. No. GTX124352

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IHC-Wm, IHC

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Tubg1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
IHC 1:100-1:1000
Not tested in other applications.

Positive Control

zebrafish muscle , whole zebrafish

Predict Reactivity

Human (100%)| Mouse (99%)| Rat (99%)| Zebrafish (98%)| Bovine (100%)| Dog (100%)| Xenopus tropicalis (99%)| Chimpanzee (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Tubg1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175042

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin, gamma 1 , tubgl , zgc:55894

Database

Research Area

DATA IMAGES

Anti-Tubg1 antibody used in Immunohistochemistry (IHC). GTX124352

GTX124352 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo, using tubgl (GTX124352) antibody at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-Tubg1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124352

GTX124352 IHC-Wm Image

Tubg1 antibody detects Tubg1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Tubg1 antibody (GTX124352) dilution: 1:100.

Anti-Tubg1 antibody used in Western Blot (WB). GTX124352

GTX124352 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubg1 antibody (GTX124352) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tubg1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124352

GTX124352 IHC-P Image

Immunohistochemical analysis of paraffin-embedded sections of zebrafish neurons, using tubgl (GTX124352) antibody at 1:300 dilution.

REFERENCE

Application Reference

Lindsey BW et al. J Comp Neurol 2012; 520 (10) : 2275-316 The cellular composition of neurogenic periventricular zones in the adult zebrafish forebrain.
Application : IHC-Fr
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169