GeneTex
United States (US)

Tubulin (Acetyl Lys40) antibody [6-11B-1]

GTX16292_20170605_ICCIF_18121410_573.jpg
GTX16292_20170605_WB_18121410_669.jpg

Cat No. GTX16292

Host Mouse
Clonality Monoclonal
Clone Name 6-11B-1
Isotype IgG2b
Application WB, ICC/IF, IHC-Wm, Dot, ELISA, EM, RIA
Reactivity Human, Mouse, Rat, Zebrafish, Bovine, Dog, Hamster, Chicken, Pig, Monkey, Frog
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2,000
ICC/IF Assay dependent
IHC-Wm Assay dependent
Dot Assay dependent
ELISA Assay dependent
EM Assay dependent
RIA Assay dependent
Not tested in other applications.

Positive Control

bovine, rat brain

Specificity/Sensitivity

The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila and yeast. The antibody has been used to detect acetylated α-tubulins in several organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it is in the kangaroo rat epithelial-like cell line PtK2.
PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Sea Urchin

Immunogen

acetylated α-tubulin from the outer arm of sea urchin sperm axonemes.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tubulin, alpha 4a Antibody , TUBA4A Antibody , H2-ALPHA Antibody , TUBA1 Antibody

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq, Jul 2008]
DATA IMAGES
GTX16292_20170605_ICCIF_18121410_573.jpg

GTX16292 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX16292 Tubulin (Acetyl Lys40) antibody [6-11B-1] at 1:2000 (red) with DAPI(blue). Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100.

GTX16292_20170605_WB_18121410_669.jpg

GTX16292 WB Image

WB analysis of (1)HeLa (2) U87 (3) COS7 (4) P19 (5) rat2 (6) CHO (7) MDBK (8) MDCK cells using GTX16292 Tubulin (Acetyl Lys40) antibody [6-11B-1] at 1:2,000.

REFERENCE
Package List Price ($)
$ 339