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Tubulin (Acetyl Lys40) antibody [6-11B-1]

Anti-Tubulin (Acetyl Lys40) antibody [6-11B-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16292
Anti-Tubulin (Acetyl Lys40) antibody [6-11B-1] used in Western Blot (WB). GTX16292

Cat. No. GTX16292

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6-11B-1

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-Wm, Dot, ELISA, EM, RIA

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Dog, Hamster, Chicken, Pig, Monkey, Frog
Package
100 μl ($449)
See all Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2,000
ICC/IF Assay dependent
IHC-Wm Assay dependent
Dot Assay dependent
ELISA Assay dependent
EM Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

bovine, rat brain

Product Note

The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila and yeast. The antibody has been used to detect acetylated α-tubulins in several organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it is in the kangaroo rat epithelial-like cell line PtK2.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Sea Urchin

Immunogen

acetylated α-tubulin from the outer arm of sea urchin sperm axonemes.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_2887530

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin alpha 4a , ALS22 , H2-ALPHA , TUBA1

Cellular Localization

Cytoplasm, cytoskeleton

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Tubulin (Acetyl Lys40) antibody [6-11B-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16292

GTX16292 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX16292 Tubulin (Acetyl Lys40) antibody [6-11B-1] at 1:2000 (red) with DAPI(blue). Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100.

Anti-Tubulin (Acetyl Lys40) antibody [6-11B-1] used in Western Blot (WB). GTX16292

GTX16292 WB Image

WB analysis of (1)HeLa (2) U87 (3) COS7 (4) P19 (5) rat2 (6) CHO (7) MDBK (8) MDCK cells using GTX16292 Tubulin (Acetyl Lys40) antibody [6-11B-1] at 1:2,000.

REFERENCE

Application Reference

Huang PY et al. Am J Cancer Res 2022; 12 (2) : 873-892 A novel HDAC11 inhibitor potentiates the tumoricidal effects of cordycepin against malignant peripheral nerve sheath tumor through the Hippo signaling pathway.
Application : WB
Reactivity : Human
Wen-Jui Lee et al. Molecules 2021; 26 (21) Type-3 Hyaluronan Synthase Attenuates Tumor Cells Invasion in Human Mammary Parenchymal Tissues.
Application : ICC/IF
Reactivity : Human
Nelson HM et al. Dev Dyn 2019; Structure, development, and functional morphology of the cement gland of the giant danio, Devario malabaricus.
Application : IHC-Wm
Reactivity : Zebrafish
Sanchez E et al. Mol Neurobiol 2018; (Epub) Pla2g6 Deficiency in Zebrafish Leads to Dopaminergic Cell Death, Axonal Degeneration, Increased β-Synuclein Expression, and Defects in Brain Functions and Pathways.
Application : IHC-Wm
Reactivity : Zebrafish
Yang H et al. Cytoskeleton (Hoboken) 2011; 68 (3) : 175-87 Megakaryocyte lineage-specific class VI β-tubulin suppresses microtubule dynamics, fragments microtubules, and blocks cell division.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 449