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Tubulin antibody [YL1/2]

Anti-Tubulin antibody [YL1/2] used in Western Blot (WB). GTX76511
Anti-Tubulin antibody [YL1/2] used in Western Blot (WB). GTX76511

Cat. No. GTX76511

Reference 15

Host

 Rat

Clonality

 Monoclonal

Clone Name

 YL1/2

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, IP, ELISA, RIA

Reactivity

 Human, Mouse, Rat, Yeast, Drosophila, Bovine, Dog, Pig, Xenopus laevis, Arabidopsis thaliana, Ashbya, Caenorhabditis elegans, Dendraster excentricus, Dictyostelium discoideum, Hemicentrotus pulcherrimus, Pig-tailed macaque, Pleurobrachia, Potato, Potorous tridactylus/Potoroo, Rhodnius prolixus, Silk worm, Strongylocentrotus purpuratus, Trypanosoma brucei
Package
100 μg ($399)
See all Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
ELISA 1/100-1/1,000
RIA Assay dependent
Not tested in other applications.

Product Note

This antibody recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin) (Wehland et al. 1983).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Yeast tubulin.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_378784

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Tubulin antibody [YL1/2] used in Western Blot (WB). GTX76511

GTX76511 WB Image

WB analysis of C6 rat glioma tissue lysate using GTX76511 Tubulin antibody [YL1/2].

Anti-Tubulin antibody [YL1/2] used in Western Blot (WB). GTX76511

GTX76511 WB Image

The data was published in the 2016 in Cell Death Discov. PMID: 27551500

REFERENCE

Application Reference

Yang Y et al. BMC Biol 2023; 21 (1) : 285 Follicular fluid C3a-peptide promotes oocyte maturation through F-actin aggregation.
Michelle Joy-Immediato et al. Front Cell Dev Biol 2021; 9 : 669086 Junctional ER Organization Affects Mechanotransduction at Cadherin-Mediated Adhesions.
Application : ICC/IF
Reactivity : Mouse
Romina Ulloa et al. Front Immunol 2021; 12 : 801164 B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction.
Application : WB
Reactivity : Mouse
Zhang M et al. Front Neurosci 2021; 15 : 709684 Long Non-coding RNA T-uc.189 Modulates Neural Progenitor Cell Fate by Regulating Srsf3 During Mouse Cerebral Cortex Development.
Application : WB
Reactivity : Mouse
Tanji K et al. Biochem Biophys Res Commun 2021; 537 : 85-92 Novel tankyrase inhibitors suppress TDP-43 aggregate formation.
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REVIEW

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Application Reference
Yang Y et al. BMC Biol 2023; 21 (1):285 Follicular fluid C3a-peptide promotes oocyte maturation through F-actin aggregation.
Michelle Joy-Immediato et al. Front Cell Dev Biol 2021; 9:669086 Junctional ER Organization Affects Mechanotransduction at Cadherin-Mediated Adhesions.
Application : ICC/IF
Reactivity : Mouse
Romina Ulloa et al. Front Immunol 2021; 12:801164 B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction.
Application : WB
Reactivity : Mouse
Zhang M et al. Front Neurosci 2021; 15:709684 Long Non-coding RNA T-uc.189 Modulates Neural Progenitor Cell Fate by Regulating Srsf3 During Mouse Cerebral Cortex Development.
Application : WB
Reactivity : Mouse
Tanji K et al. Biochem Biophys Res Commun 2021; 537:85-92 Novel tankyrase inhibitors suppress TDP-43 aggregate formation.
Mock ED et al. Nat Chem Biol 2020; 16 (6):667-675 Discovery of a NAPE-PLD inhibitor that modulates emotional behavior in mice.
Application : WB
Reactivity : Human
Liang S et al. Oncol Lett 2020; 19 (4):3197-3204 METTL3 serves an oncogenic role in human ovarian cancer cells partially via the AKT signaling pathway.
Application : WB
Reactivity : Human
Selvam K et al. J Mol Biol 2019; (Epub) Evidence that Moderate Eviction of Spt5 and Promotion of Error-Free Transcriptional Bypass by Rad26 Facilitates Transcription Coupled Nucleotide Excision Repair.
Application : WB
Reactivity : Yeast
Chien T et al. Mol Psychiatry 2018; (Epub) GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.
Application : WB
Reactivity : Rat
Hsieh CL et al. Sci Rep 2017; 7 (1):9065 Reactive oxygen species-mediated switching expression of MMP-3 in stromal fibroblasts and cancer cells during prostate cancer progression.
Application : WB
Reactivity : Human
Chien JY et al. Cell Death Discov 2016; 2:16006 αTAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells.
Application : WB
Reactivity : Human
Rongfang Liu et al. European Journal of Pharmacology 2016; 770:70-77 The role of the C-terminus of the human hydroxycarboxylic acid receptors 2 and 3 in G protein activation using Gα-engineered yeast cells.
Application : WB
Reactivity : Yeast
Liu R et al. Biochem Pharmacol 2015; 95 (4):290-300 Scanning mutagenesis in a yeast system delineates the role of the NPxxY(x)5,6F motif and helix 8 of the adenosine A2B receptor in G protein coupling.
Application : WB
Reactivity : Yeast
Chen X et al. PLoS One 2009; 4 (4):e5267 Rpb1 sumoylation in response to UV radiation or transcriptional impairment in yeast.
Application : WB
Reactivity : Yeast
den Dulk B et al. DNA Repair (Amst) 2006; 5 (6):683-92 Rad33, a new factor involved in nucleotide excision repair in Saccharomyces cerevisiae.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399