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Tubulin antibody

Anti-Tubulin antibody used in Western Blot (WB). GTX11270
Anti-Tubulin antibody used in Western Blot (WB). GTX11270

Cat. No. GTX11270

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Mouse, Chicken
Package
200 μl ($649)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200
ICC/IF 1:80
Not tested in other applications.

Positive Control

chicken gizzard, mouse brain

Product Note

The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

microtubule proteins from brains of 13 day old chicken embryos

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta, that assemble into microtubules. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Both alpha and beta tubulins consist of various isotypes. In addition, both undergo post-translational modifications, including acetylation, phosphorylation detyrosination, polyglutamylation, and polyglycylation. Polyglutamylation of tubulin consists of the addition of a lateral chain of glutamyl units on the C-terminal region of tubulin polypeptides. This modification was shown to regulate the interaction between tau, one of the major neuronal microtubule associated proteins, and tubulin. Furthermore, it was shown that injection of monoclonal antibodies specific to the polyglutamylated form of tubulin into HeLa cells caused the disappearance of centrioles, an organelle known to have the most stable microtubules. The detection, localization, and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions. Antibodies reacting specifically with the modified forms of alpha and beta tubulin isotypes serve as essential tools in the detection and study of the functional significance of these molecules.

Research Area

DATA IMAGES

Anti-Tubulin antibody used in Western Blot (WB). GTX11270

GTX11270 WB Image

WB analysis of chicken gizzard extract using GTX11270 Tubulin antibody at 1:50.

Anti-Tubulin antibody used in Western Blot (WB). GTX11270

GTX11270 WB Image

WB analysis of mouse brain extract using GTX11270 Tubulin antibody.
Lane 1 : Antibody dilution 1:500
Lane 2 : Negative control (without primary antibody)

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 649