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United States (US)

Tween 20 (50%)

Cat No. GTX30962

Reference 1

Application

 WB, ICC/IF, ELISA, IHC
Package
50 ml ($89)
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PRODUCT

Summary

Tween 20 (50%)

APPLICATION

Application Note

For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml).
Washing buffer use 1:500 (1 ml into 499 ml).
The optimum dilution should be determined by the individual lab.

PROPERTIES

Buffer

This detergent is supplied as 50% in water with 0.05 % preservative.

Storage

Store at room temperature.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Non ionic detergents like Tween 20 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tween 20 or Polysorbate 20 PEG(20) sorbitan monolaurate, lowers surface tension of water.

REFERENCE

Application Reference

Webb SJ et al. Environ Sci Technol 2014; Establishment, Characterization, and Toxicological Application of Loggerhead Sea Turtle (Caretta caretta) Primary Skin Fibroblast Cell Cultures.
Application : ICC/IF
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Package List Price ($)
$ 89