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Twist1/2 antibody

Overexpression
GTX127310_41430_IHC_M_18121410_567.jpg
GTX127310_41430_IHC_R_18121410_466.jpg
GTX127310_41220_20151029_WB_18121410_134.jpg
GTX127310_41220_20151029_WB_B_18121410_115.jpg
GTX127310_41430_IHC_M_2_18121410_136.jpg

Cat No. GTX127310

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Raji , THP-1 , HL-60 , TWIST1 transfected 293T lysates , human TWIST1-transfected 293T , Jurkat , *OECM1 , *CL1-5 , *Toledo , *SU-DHL-5 , *HT , *DB , *RL95-2 , *MDA-MB-231

Predict Reactivity

Bovine, Chicken, Japanese Medaka, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Twist1/2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ACS3 antibody, B-HLH DNA binding protein antibody, BPES2 antibody, BPES3 antibody, CRS1 antibody, DERMO1 antibody, H-twist antibody, MGC117334 antibody, SCS antibody, TWIST antibody, TWIST homolog of drosophila antibody, TWIST1 antibody, TWIST2 antibody, bHLHa38 antibody, bHLHa39 antibody, class A basic helix-loop-helix protein 38 antibody, class A basic helix-loop-helix protein 39 antibody, dermis-expressed protein 1 antibody, twist homolog 2 (Drosophila) antibody, twist-related bHLH protein Dermo1 antibody, twist-related protein 1 antibody, twist-related protein 2 antibody

Cellular Localization

Nucleus

Background

This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]
DATA IMAGES
GTX127310_41430_IHC_M_18121410_567.jpg

GTX127310 IHC-P Image

Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Twist1/2 antibody (GTX127310) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX127310_41430_IHC_R_18121410_466.jpg

GTX127310 IHC-P Image

Twist1/2 antibody detects Twist1/2 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Twist1/2 antibody (GTX127310) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX127310_41220_20151029_WB_18121410_134.jpg

GTX127310 WB Image

Twist1/2 antibody detects Twist1/2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127310_41220_20151029_WB_B_18121410_115.jpg

GTX127310 WB Image

Overexpression

Twist1/2 antibody detects Twist1 protein by western blot analysis. Non-transfected (-) and Twist1-transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127310_41430_IHC_M_2_18121410_136.jpg

GTX127310 IHC-P Image

Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Twist1/2 antibody (GTX127310) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

Twist1/2 antibody Cat No. GTX127310

Rating ( Average 4.6 based on 9 users reviews)
Application
Western Blot(WB) ( Average 4.6 based on 9 users reviews)
Date : Anonymous submitted on 01-Nov-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : KLE cells
Amount used : 10 μg
Blocking : PVDF Blocking Reagent for Can Get Signal, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Lane Description : Lane 1: Control, Lane 2: TGF-β 5ng/mL treatment
Date : Anonymous submitted on 18-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 1* 10^5 cells
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 29-Apr-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC3 cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 24Hr
Date : Anonymous submitted on 24-Jul-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Date : Anonymous submitted on 04-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : RL95-2 cells
Amount used : 120μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 07-Apr-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : Toledo cells, SU-DHL-5 cells, HT cells, DB cells, transfected hTWIST1 in HEK293 cells
Amount used : 30μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Date : Anonymous submitted on 11-Jun-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC9 cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:4000, 25°C, 4Hr
Date : Anonymous submitted on 22-Nov-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : CL1-5 cells
Amount used : 40μg
Blocking : 5% milk, 25°C, 3Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 31-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : OECM1 cells
Amount used : 40μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169