APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , THP-1 , HL-60 , human Twist1-transfected 293T
Predict Reactivity
Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.67 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Twist1/2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885640
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ACS3 antibody , B-HLH DNA binding protein antibody , BPES2 antibody , BPES3 antibody , CRS1 antibody , DERMO1 antibody , H-twist antibody , MGC117334 antibody , SCS antibody , TWIST antibody , TWIST homolog of drosophila antibody , TWIST1 antibody , TWIST2 antibody , bHLHa38 antibody , bHLHa39 antibody , class A basic helix-loop-helix protein 38 antibody , class A basic helix-loop-helix protein 39 antibody , dermis-expressed protein 1 antibody , twist homolog 2 (Drosophila) antibody , twist-related bHLH protein Dermo1 antibody , twist-related protein 1 antibody , twist-related protein 2 antibody
Cellular Localization
Nucleus
Background
This gene encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. This gene is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism. [provided by RefSeq, Aug 2017]
Research Area
DATA IMAGES
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GTX127310 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX127310 IHC-P Image
Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Twist1/2 antibody (GTX127310) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127310 IHC-P Image
Twist1/2 antibody detects Twist1/2 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Twist1/2 antibody (GTX127310) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127310 WB Image
Twist1/2 antibody detects Twist1 protein by western blot analysis. Non-transfected (-) and Twist1-transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX127310 IHC-P Image
Twist1/2 antibody detects Twist1/2 protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Twist1/2 antibody (GTX127310) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX127310 WB Image
Twist1/2 antibody detects Twist1/2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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|
GTX127310 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Twist1/2 antibody (GTX127310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
Twist1/2 antibody
Cat. No. GTX127310
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Rating
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( Average 4.8 based on 21 users reviews)
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Application
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Western Blot(WB)
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( Average 4.8 based on 20 users reviews)
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Immunohistochemistry(IHC)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 02-May-2023
Application Tested :
WB
Sample Species :
Hu
Sample :
MDA-MB-231 cell lysates with/without treatment
Amount used :
40μg
Blocking :
5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 08-Aug-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
Huh7 cells
Amount used :
30μg
Blocking :
5% non-fat milk, BSA in TBST, 4°C, 1Hr
Primary Antibody :
1:1000, 4°C, > 12Hr
Date :
Anonymous submitted on 19-Apr-2022
Application Tested :
WB
Sample Species :
Hu
Sample :
HSC-3 cells with/without drug treatment
Amount used :
30μg
Blocking :
5% non-fat milk in TBST, 30°C, 1Hr
Primary Antibody :
1:1000, 4°C, 18Hr
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
IHC
Sample Species :
Dm
Figure Note :
The data was peer reviewed and published in the journal Sci Adv in 2019.
PMID: 31309148
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Oncotarget in 2016.
PMID: 26840566
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2019.
PMID: 30816208
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2019.
PMID: 30816208
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Biomolecules in 2019.
PMID: 31877715
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Front Oncol in 2019.
PMID: 31440468
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2015.
PMID: 26136074
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal PLoS One in 2017.
PMID: 29182685
Date :
Anonymous submitted on 18-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2017.
PMID: 28134285
Date :
Anonymous submitted on 01-Nov-2017
Application Tested :
WB
Sample Species :
Hu
Sample :
KLE cells
Amount used :
10 μg
Blocking :
PVDF Blocking Reagent for Can Get Signal, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 24Hr
Lane Description :
Lane 1: Control, Lane 2: TGF-β 5ng/mL treatment
Date :
Anonymous submitted on 18-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells
Amount used :
1* 10^5 cells
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 29-Apr-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
PC3 cells
Amount used :
30μg
Blocking :
5% milk in PBST, 25°C, 1Hr
Primary Antibody :
1:500, 4°C, 24Hr
Date :
Anonymous submitted on 24-Jul-2015
Application Tested :
WB
Sample Species :
Hu
Sample :
MDA-MB-231 cells
Amount used :
30μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 24Hr
Date :
Anonymous submitted on 04-Aug-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
RL95-2 cells
Amount used :
120μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 07-Apr-2014
Application Tested :
WB
Sample Species :
Hu
Sample :
Toledo cells, SU-DHL-5 cells, HT cells, DB cells, transfected hTWIST1 in HEK293 cells
Amount used :
30μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr
Date :
Anonymous submitted on 11-Jun-2013
Application Tested :
WB
Sample Species :
Hu
Sample :
PC9 cells
Amount used :
30μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:4000, 25°C, 4Hr
Date :
Anonymous submitted on 22-Nov-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
CL1-5 cells
Amount used :
40μg
Blocking :
5% milk, 25°C, 3Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Date :
Anonymous submitted on 31-Oct-2012
Application Tested :
WB
Sample Species :
Hu
Sample :
OECM1 cells
Amount used :
40μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
