Twist1 antibody [10E4E6]

Name: Twist1 antibody [10E4E6]
Brand: GeneTex
SKU: GTX60776
Price: 399.00 USD

Anti-Twist1 antibody [10E4E6] used in Flow cytometry (FACS). GTX60776

Anti-Twist1 antibody [10E4E6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60776

Anti-Twist1 antibody [10E4E6] used in Western Blot (WB). GTX60776

Anti-Twist1 antibody [10E4E6] used in IHC (Paraffin sections) (IHC-P). GTX60776

Anti-Twist1 antibody [10E4E6] used in ELISA (ELISA). GTX60776

Cat. No. GTX60776

Reference 6

Host	Mouse
Clonality	Monoclonal
Clone Name	10E4E6
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, IP, ELISA
Reactivity	Human, Mouse
Package	100 μg ($399)

See all Twist1 products

View product citations for antibody GTX60776 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
IP	Assay dependent
ELISA	1/10000

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human TWIST1 (AA: 9-74) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2888002

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

twist family bHLH transcription factor 1 , ACS3 , BPES2 , BPES3 , CRS , CRS1 , CSO , SCS , SWCOS , TWIST , bHLHa38

Cellular Localization

Nucleus

Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7291 TWIST1
UniProt: Q15672 TWIST1

Research Area

DATA IMAGES

GTX60776 FACS Image

FACS analysis of HeLa cells using GTX60776 Twist1 antibody [10E4E6].
Green : Twist1
Red : negative control

GTX60776 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60776 Twist1 antibody [10E4E6].
Green : Twist1
Blue: DRAQ5 fluorescent DNA dye

GTX60776 WB Image

WB analysis of HEK293 (1) and TWIST1 (AA: 9-74)-hIgGFc transfected HEK293 (2) cell lysate using GTX60776 Twist1 antibody [10E4E6].

GTX60776 IHC-P Image

IHC-P analysis of cervical cancer tissue using GTX60776 Twist1 antibody [10E4E6].

GTX60776 WB Image

WB analysis of NIH3T3 (1), Jurkat (2), HeLa (3), A549 (4), Raji (5) and OCM-1 (6) cell lysate using GTX60776 Twist1 antibody [10E4E6].

GTX60776 ELISA Image

ELISA analysis of antigen using GTX60776 Twist1 antibody [10E4E6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng