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Twist1 antibody [N1C3]

Overexpression
Anti-Twist1 antibody [N1C3] used in Western Blot (WB). GTX100621
Anti-Twist1 antibody [N1C3] used in Western Blot (WB). GTX100621

Cat. No. GTX100621

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Twist1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Flag-human TWIST1-transfected HeLa cells

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Twist1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952452

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

twist family bHLH transcription factor 1 , ACS3 , BPES2 , BPES3 , CRS , CRS1 , CSO , SCS , SWCOS , TWIST , bHLHa38

Cellular Localization

Nucleus

Background

Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Twist1 antibody [N1C3] used in Western Blot (WB). GTX100621

GTX100621 WB Image

Twist1 antibody [N1C3] detects Twist1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Flag-human TWIST1-transfected HeLa cells
12 % SDS-PAGE
Twist1 antibody [N1C3] (GTX100621) dilution: 1:10000

Anti-Twist1 antibody [N1C3] used in Western Blot (WB). GTX100621

GTX100621 WB Image

Overexpression

The data was published in the journal PLoS One in 2017. PMID: 28683123

REFERENCE

Application Reference

Lv F et al. Eur J Pharmacol 2021; 900 : 174069 Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3β/ZEB1 pathway.
Lee AF et al. PLoS One 2017; 12 (7) : e0180383 Reverse epithelial-mesenchymal transition contributes to the regain of drug sensitivity in tyrosine kinase inhibitor-resistant non-small cell lung cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169