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Tyrosine Hydroxylase antibody [185]

Anti-Tyrosine Hydroxylase antibody [185] used in IHC (Paraffin sections) (IHC-P). GTX10372
Anti-Tyrosine Hydroxylase antibody [185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10372

Cat No. GTX10372

Host Mouse
Clonality Monoclonal
Clone Name 185
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
50 μl ($319)
APPLICATION

Application Note

IHC-Fr: Use at a dilution of 1/20 - 1/40. WB: Use at a dilution of 1/25 - 1/50. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

59 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Preservative: 15mM Sodium Azide; Constituents: Reconstituted supernantant.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Mouse

Immunogen

Recombinant protein, C-terminal portion of mouse sequence.

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine Hydroxylase , Dyt14 , Dyt5B , Tyh , Th

Background

Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.

Database

Research Area

DATA IMAGES
Anti-Tyrosine Hydroxylase antibody [185] used in IHC (Paraffin sections) (IHC-P). GTX10372

GTX10372 IHC-P Image

TH staining of human mid-brain. Note cytoplasmic staining of catecholaminergic cells and their processes. Paraffin section (Peroxidase substrate: nickel DAB, Counterstain: eosin).

Anti-Tyrosine Hydroxylase antibody [185] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10372

GTX10372 ICC/IF Image

Immunoperoxidase (A and C) and immunofluorescent (B, D–H) labeling of intrinsic cardiac adrenergic (ICA) cells in human hearts are shown. ICA cells expressing tyrosine hydroxylase (TH) immunoreactivity (red) are distributed diffusely throughout the left ventricular (LV) myocardium. Perivascular location is a frequent feature of ICA cells. C, arrow: terminal arteriole. E: abundant ICA cells in the smooth muscle layers of epicardial circumflex coronary artery. TH-expressing sympathetic nerve fibers (D and G, arrows) can occasionally be seen in the field. B and D, insets: magnified ICA cell images (arrows). TH immunoreactivity (green) was identified in ICA cells and sympathetic nerve fibers in the sinoatrial nodal tissue (G). ICA cells are seen in transplanted human LV tissue (H). Scale bars = 10 µm, except in B (20 µm)

REFERENCE
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Package List Price ($)
$ 319