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Tyrosine Hydroxylase antibody, C-term

Anti-Tyrosine Hydroxylase antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89578
Anti-Tyrosine Hydroxylase antibody, C-term used in Western Blot (WB). GTX89578
Anti-Tyrosine Hydroxylase antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89578

Cat. No. GTX89578

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human
Package
100 μg ($399)
See all Tyrosine Hydroxylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-4μg/ml
IHC-Fr 0.1-0.3μg/ml

Note :

IHC-Fr
Human Hypothalamus shows staining of neurons and their processes.
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89578-PEP).

Product Note

This antibody is expected to recognise all three reported isoforms (as represented by NP_954986.2; NP_000351.2; NP_954987.2).

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-VQDELDTLAHAL, from the C Terminus of the protein sequence according to NP_954986.2; NP_000351.2; NP_954987.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine hydroxylase , DYT14 , DYT5b , TYH

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Tyrosine Hydroxylase antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89578

GTX89578 IHC-Fr Image

IHC-Fr analysis of immersion-fixed (4% PFA) human hypothalamus using GTX89578 Tyrosine Hydroxylase antibody, C-term.

Anti-Tyrosine Hydroxylase antibody, C-term used in Western Blot (WB). GTX89578

GTX89578 WB Image

WB analysis of human cerebral cortex lysate using GTX89578 Tyrosine Hydroxylase antibody, C-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Tyrosine Hydroxylase antibody, C-term used in IHC (Frozen sections) (IHC-Fr). GTX89578

GTX89578 IHC-Fr Image

IHC-Fr analysis of PFA-perfused human hypothalamic supraoptic (SO), paraventricular (Pa) and periventricular (Pe) nuclei using GTX89578 Tyrosine Hydroxylase antibody, C-term.
Antigen retrieval : citrate buffer pH 6 at 80C for 30min
Dilution : 0.1μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399