GeneTex
United States (US)

Tyrosine Hydroxylase antibody [GT234]

GTX634481_43143_20181005_IHC-P-FL_M_18121411_245.jpg
GTX634481_43143_20180518_WB_M_brain_18121411_426.jpg
GTX634481_43143_20180713_IHC-P-FL_M_18121411_878.jpg
GTX634481_43143_20180316_WB_R_brain_18121411_928.jpg

Cat No. GTX634481

Host Mouse
Clonality Monoclonal
Clone Name GT234
Isotype IgG1
Application WB, IHC-P
Reactivity Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Zebrafish, Bovine, Dog, Chicken, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine Hydroxylase,Dyt14,Dyt5B,Tyh,Th

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Research Area

DATA IMAGES
GTX634481_43143_20181005_IHC-P-FL_M_18121411_245.jpg

GTX634481 IHC-P Image

Tyrosine Hydroxylase antibody [GT234] detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX634481_43143_20180518_WB_M_brain_18121411_426.jpg

GTX634481 WB Image

Mouse tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634481_43143_20180713_IHC-P-FL_M_18121411_878.jpg

GTX634481 IHC-P Image

Tyrosine Hydroxylase antibody [GT234] detects Tyrosine Hydroxylase protein at cytoplasm of dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse midbrain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX634481_43143_20180316_WB_R_brain_18121411_928.jpg

GTX634481 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169