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Tyrosine Hydroxylase antibody [GT234]

Anti-Tyrosine Hydroxylase antibody [GT234] used in IHC (Paraffin sections) (IHC-P). GTX634481
Anti-Tyrosine Hydroxylase antibody [GT234] used in Western Blot (WB). GTX634481
Anti-Tyrosine Hydroxylase antibody [GT234] used in IHC (Paraffin sections) (IHC-P). GTX634481
Anti-Tyrosine Hydroxylase antibody [GT234] used in Western Blot (WB). GTX634481

Cat No. GTX634481

Host

Mouse

Clonality

Monoclonal

Clone Name

GT234

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

mouse brain , rat brain

Predict Reactivity

Human, Zebrafish, Bovine, Dog, Chicken, Japanese Medaka, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2802158

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tyrosine hydroxylase , DYT14 , DYT5b , TYH

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Tyrosine Hydroxylase antibody [GT234] used in IHC (Paraffin sections) (IHC-P). GTX634481

GTX634481 IHC-P Image

Tyrosine Hydroxylase antibody [GT234] detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody [GT234] used in Western Blot (WB). GTX634481

GTX634481 WB Image

Mouse tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Tyrosine Hydroxylase antibody [GT234] used in IHC (Paraffin sections) (IHC-P). GTX634481

GTX634481 IHC-P Image

Tyrosine Hydroxylase antibody [GT234] detects Tyrosine Hydroxylase protein at cytoplasm of dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse midbrain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody [GT234] used in Western Blot (WB). GTX634481

GTX634481 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [GT234] (GTX634481) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

Tyrosine Hydroxylase antibody [GT234] Cat No. GTX634481

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 04-Jun-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : Mouse Brain, 250μm slice, coronal section
Fixation : 4% PFA in PBS, 4°C, 16Hr
Permeabilization /Antigen retrieval : 2% Triton X-100 in PBS, 25°C, 48Hr
Primary Antibody : 1:100, 4°C, 48Hr
Figure Note : This antibody was used to detect Tyrosine Hydroxylase expression in mice brain tissue (~250μm thickness) by Immunofluorescence (IF). The figure shown dopamine neurons of the substantia nigra project to the striatum.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169