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Tyrosine Hydroxylase antibody [N1C1]

GTX102424_39974_20160921_IHC-Fr_19011506_720.jpg
GTX102424_39974_20160830_IHC-Fr_19011506_269.jpg
GTX102424_39974_20181005_IHC-P-FL_M_19011506_198.jpg
GTX102424_39974_20180403_IHC-P_M_1_19011506_588.jpg
GTX102424_39974_20171020_WB_19011506_519.jpg
GTX102424_39974_20161205_IHC-P_R_19011506_326.jpg
GTX102424_39974_20160906_IHC-Wm_Z_1_19011506_819.jpg
GTX102424_39974_20150410_IFA_19011506_133.jpg
GTX102424_39974_20170713_WB_R_brain_19011506_227.jpg
GTX102424_39974_20180403_IHC-P_M_19011506_870.jpg
GTX102424_39974_20160204_WB_M_brain_19011506_925.jpg
GTX102424_39974_20160906_IHC-Wm_Z_19011506_819.jpg
GTX102424_39974_20180403_IHC-P_M_2_19011506_911.jpg
GTX102424_39974_20150410_IFA_2_19011506_195.jpg

Cat No. GTX102424

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm
Reactivity Human, Mouse, Rat, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

IMR32 , mouse brain , rat brain
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine Hydroxylase,Dyt14,Dyt5B,Tyh,Th

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102424_39974_20160921_IHC-Fr_19011506_720.jpg

GTX102424 IHC-Fr Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:250.
Red: SOX2, stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX102424_39974_20160830_IHC-Fr_19011506_269.jpg

GTX102424 IHC-Fr Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX102424_39974_20181005_IHC-P-FL_M_19011506_198.jpg

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424_39974_20180403_IHC-P_M_1_19011506_588.jpg

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424_39974_20171020_WB_19011506_519.jpg

GTX102424 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102424_39974_20161205_IHC-P_R_19011506_326.jpg

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424_39974_20160906_IHC-Wm_Z_1_19011506_819.jpg

GTX102424 IHC-Wm Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tyrosine Hydroxylase antibody [N1C1] (GTX102424) dilution: 1:100.

GTX102424_39974_20150410_IFA_19011506_133.jpg

GTX102424 ICC/IF Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX102424_39974_20170713_WB_R_brain_19011506_227.jpg

GTX102424 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX102424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102424_39974_20180403_IHC-P_M_19011506_870.jpg

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424_39974_20160204_WB_M_brain_19011506_925.jpg

GTX102424 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000.

GTX102424_39974_20160906_IHC-Wm_Z_19011506_819.jpg

GTX102424 IHC-Wm Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tyrosine Hydroxylase antibody [N1C1] (GTX102424) dilution: 1:100.

GTX102424_39974_20180403_IHC-P_M_2_19011506_911.jpg

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424_39974_20150410_IFA_2_19011506_195.jpg

GTX102424 ICC/IF Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REVIEW

Tyrosine Hydroxylase antibody [N1C1] Cat No. GTX102424

Rating ( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 28-Jun-2016
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Retina tissue
Blocking : 20% normal donkey serum and 0.3% Triton X-100 in PBS, 25°C, 1Hr
Fixation : 4%Paraformaldehyde+0.01%Glutaraldehyde in PBS
Primary Antibody : 1:250, 4°C, 24Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169