Tyrosine Hydroxylase antibody [N1C1]

Name: Tyrosine Hydroxylase antibody [N1C1]
Brand: GeneTex
SKU: GTX102424
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-Tyrosine Hydroxylase antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX102424

Anti-Tyrosine Hydroxylase antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX102424

Anti-Tyrosine Hydroxylase antibody [N1C1] used in Western Blot (WB). GTX102424

Anti-Tyrosine Hydroxylase antibody [N1C1] used in IHC (Whole mount) (IHC-Wm). GTX102424

Anti-Tyrosine Hydroxylase antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102424

Cat. No. GTX102424

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all Tyrosine Hydroxylase products

View product citations for antibody GTX102424 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000
IHC-Wm	1:100-1:500

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

IMR32

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038197

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine hydroxylase , DYT14 , DYT5b , TYH

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Database

Gene ID: 7054 TH
UniProt: P07101 TH

Research Area

DATA IMAGES

GTX102424 IHC-Fr Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:250.
Red: SOX2, stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX102424 IHC-Fr Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 IHC-Wm Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Tyrosine Hydroxylase antibody [N1C1] (GTX102424) dilution: 1:100.

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 ICC/IF Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX102424 IHC-P Image

GTX102424 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102424 IHC-P Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102424 ICC/IF Image

Tyrosine Hydroxylase antibody [N1C1] detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody [N1C1] (GTX102424) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX102424 IHC-Wm Image

GTX102424 IHC-P Image

REFERENCE

Application Reference

S Chen et al. Environ Sci Technol 2023; 57 (48) : 19407-19418 Neurotoxicity of the Cu(OH)2 Nanopesticide through Perturbing Multiple Neurotransmitter Pathways in Developing Zebrafish

Application : WB

Lin W et al. iScience 2023; 26 (4) : 106285 Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons.

Application : ICC/IF

Reactivity : Human

Submit a Reference

REVIEW

Tyrosine Hydroxylase antibody [N1C1] Cat. No. GTX102424

Rating

( Average 4.5 based on 2 users reviews)

Application

Western Blot(WB)		( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)		( Average 4 based on 1 users reviews)

Date : Anonymous submitted on 16-Sep-2021

Score :

Application Tested : WB

Sample Species : Hu

Sample : IMR32 Neuroblast (ATCC CCL-127)

Amount used : 35μg

Blocking : 5% BSA in PBST, 25°C, 1Hr

Primary Antibody : 0.57μg/ml, 4°C, 16Hr

Lane Description :
Lane 1 : IMR-32 (ATCC CCL-127) as control.
Lane 2-4 : IMR-32 (ATCC CCL-127) were differentiated and harvested on different days.

Date : Anonymous submitted on 28-Jun-2016

Score :

Application Tested : IHC-P

Sample Species : Ms

Sample : Retina tissue

Blocking : 20% normal donkey serum and 0.3% Triton X-100 in PBS, 25°C, 1Hr

Fixation : 4%Paraformaldehyde+0.01%Glutaraldehyde in PBS

Primary Antibody : 1:250, 4°C, 24Hr

Submit a Review

Application Reference

S Chen et al. Environ Sci Technol 2023; 57 (48):19407-19418 Neurotoxicity of the Cu(OH)2 Nanopesticide through Perturbing Multiple Neurotransmitter Pathways in Developing Zebrafish

Application : WB

Lin W et al. iScience 2023; 26 (4):106285 Dendritic spine formation and synapse maturation in transcription factor-induced human iPSC-derived neurons.

Application : ICC/IF

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package	List Price ($)
100 μl	$ 399
25 μl	$ 169
Qty

Tyrosine Hydroxylase antibody [N1C1]

Cat. No. GTX102424

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Positive Control

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Background

Database

Research Area

DATA IMAGES

GTX102424 IHC-Fr Image

GTX102424 IHC-Fr Image

GTX102424 IHC-P Image

GTX102424 WB Image

GTX102424 IHC-P Image

GTX102424 IHC-Wm Image

GTX102424 IHC-P Image

GTX102424 IHC-P Image

GTX102424 ICC/IF Image

GTX102424 IHC-P Image

GTX102424 WB Image

GTX102424 IHC-P Image

GTX102424 ICC/IF Image

GTX102424 IHC-Wm Image

GTX102424 IHC-P Image

REFERENCE

Application Reference

REVIEW

Tyrosine Hydroxylase antibody [N1C1] Cat. No. GTX102424

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.