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Tyrosine Hydroxylase antibody

Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016
Anti-Tyrosine Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016
Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016
Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016
Anti-Tyrosine Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016
Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

Cat No. GTX113016

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Vole, Calf
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , SK-N-SH , mouse brain , PC-12 , rat brain

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tyrosine hydroxylase,DYT14,DYT5b,TYH

Background

The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016

GTX113016 IHC-Fr Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Tyrosine Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX113016

GTX113016 IHC-P Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

GTX113016 WB Image

Tyrosine Hydroxylase antibody detects TH protein by western blot analysis.
A. 30 µg NT2D1 whole cell lysate/extract
B. 30 µg PC-3 whole cell lysate/extract
C. 30 µg U87-MG whole cell lysate/extract
D. 30 µg SK-N-SH whole cell lysate/extract
7.5% SDS-PAGE
Tyrosine Hydroxylase antibody (GTX113016) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

GTX113016 WB Image

Tyrosine Hydroxylase antibody detects TH protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
7.5% SDS-PAGE
Tyrosine Hydroxylase antibody (GTX113016) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016

GTX113016 IHC-Fr Image

Immunohistochemical analysis of Rat hindlimb pad skin tissue (paraformaldehyde-fixed frozen sections), using Tyrosine Hydroxylase(GTX113016) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody used in IHC (Frozen sections) (IHC-Fr). GTX113016

GTX113016 IHC-Fr Image

Immunohistochemical analysis of Rat hindlimb pad skin tissue (paraformaldehyde-fixed frozen sections), using Tyrosine Hydroxylase(GTX113016) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody used in IHC (Paraffin sections) (IHC-P). GTX113016

GTX113016 IHC-P Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:2500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Tyrosine Hydroxylase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113016

GTX113016 ICC/IF Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:400.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.

Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

GTX113016 WB Image

Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Tyrosine Hydroxylase antibody used in Western Blot (WB). GTX113016

GTX113016 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:2000.

REFERENCE
REVIEW
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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169