GeneTex
United States (US)

Tyrosine Hydroxylase (phospho Ser40) antibody

GTX16557_296_IHC_18121410_690.jpg
GTX16557_1630_WB_18121410_383.jpg

Cat No. GTX16557

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr, IHC
Reactivity Mouse, Rat, Starling, Bird
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:1000
IHC-Fr 1:1000
IHC 1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Specificity/Sensitivity

Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
PROPERTIES

Form

Liquid

Buffer

10mM HEPES pH 7.5, 0.15M NaCl, 0.1 mg/ml BSA, 50% Glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine Hydroxylase,The,Th

Background

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD]

Database

Research Area

DATA IMAGES
GTX16557_296_IHC_18121410_690.jpg

GTX16557 IHC-Fr Image

IHC-Fr analysis of retina tissue using GTX16557 Tyrosine Hydroxylase (phospho Ser40) antibody. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.
Left : pan TH antibody
Right : Tyrosine Hydroxylase (phospho Ser40) antibody

GTX16557_1630_WB_18121410_383.jpg

GTX16557 WB Image

WB analysis of recombinant phospho-TH and non-phospho-TH protein using GTX16557 Tyrosine Hydroxylase (phospho Ser40) antibody.

REFERENCE
Package List Price ($)
$ 399