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Tyrosine Hydroxylase (phospho Ser40) antibody

Anti-Tyrosine Hydroxylase (phospho Ser40) antibody used in Western Blot (WB). GTX16557
Anti-Tyrosine Hydroxylase (phospho Ser40) antibody used in IHC (Frozen sections) (IHC-Fr). GTX16557
Anti-Tyrosine Hydroxylase (phospho Ser40) antibody used in IHC (Frozen sections) (IHC-Fr). GTX16557
Anti-Tyrosine Hydroxylase (phospho Ser40) antibody used in IHC (Paraffin sections) (IHC-P). GTX16557

Cat. No. GTX16557

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Mouse, Rat, Starling, Bird, Sparrow
Package
100 μl ($479)
See all Tyrosine Hydroxylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:10 - 1:500
IHC-P Assay dependent
IHC-Fr 1:1000
IHC 1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Product Note

Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.

PROPERTIES

Form

Liquid

Buffer

10mM HEPES pH7.5, 0.15M NaCl, 0.1mg/ml BSA, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser40 of rat tyrosine hydroxylase.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_373133

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine hydroxylase , The

Background

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD]

Database

Research Area

DATA IMAGES

Anti-Tyrosine Hydroxylase (phospho Ser40) antibody used in Western Blot (WB). GTX16557

GTX16557 WB Image

WB analysis of recombinant phospho-TH and non-phospho-TH protein using GTX16557 Tyrosine Hydroxylase (phospho Ser40) antibody.

Anti-Tyrosine Hydroxylase (phospho Ser40) antibody used in IHC (Frozen sections) (IHC-Fr). GTX16557

GTX16557 IHC-Fr Image

IHC-Fr analysis of retina tissue using GTX16557 Tyrosine Hydroxylase (phospho Ser40) antibody. The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.
Left : pan TH antibody
Right : Tyrosine Hydroxylase (phospho Ser40) antibody

Anti-Tyrosine Hydroxylase (phospho Ser40) antibody used in IHC (Frozen sections) (IHC-Fr). GTX16557

GTX16557 IHC-Fr Image

The data was published in the journal PLoS One in 2012. PMID: 22724011

Anti-Tyrosine Hydroxylase (phospho Ser40) antibody used in IHC (Paraffin sections) (IHC-P). GTX16557

GTX16557 IHC-P Image

The data was published in the journal PLoS One in 2008. PMID: 18398484

REFERENCE

Application Reference

Heimovics S et al. Front Endocrinol (Lausanne) 2023; 14 : 1153085 Dehydroepiandrosterone (DHEA) increases undirected singing behavior and alters dopaminergic regulation of undirected song in non-breeding male European starlings (Sturnus vulgaris).
Ryan J.Tomm et al. Neurobiology of Aging 2018; (Epub) Effects of aging on executive functioning and mesocorticolimbic * markers in male Fischer 344 × Brown Norway rats.
Struntz KH et al. Behav Brain Res 2018; 348 : 211-218 Effects of * exposure on anxiety-like behavior in the open field test, corticosterone, and hippocampal tyrosine hydroxylase in adolescent and adult mice.
Application : WB
Reactivity : Mouse
Ellis JM et al. Brain Res 2012; Patterns of phosphorylated tyrosine hydroxylase vary with song production in female starlings.
Application : IHC-Fr
Reactivity : Starling
Matragrano LL et al. PLoS One 2012; 7 (6) : e39388 Rapid effects of hearing song on catecholaminergic activity in the songbird auditory pathway.
Application : IHC
Reactivity : Bird
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REVIEW

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Application Reference
Heimovics S et al. Front Endocrinol (Lausanne) 2023; 14:1153085 Dehydroepiandrosterone (DHEA) increases undirected singing behavior and alters dopaminergic regulation of undirected song in non-breeding male European starlings (Sturnus vulgaris).
Ryan J.Tomm et al. Neurobiology of Aging 2018; (Epub) Effects of aging on executive functioning and mesocorticolimbic * markers in male Fischer 344 × Brown Norway rats.
Struntz KH et al. Behav Brain Res 2018; 348:211-218 Effects of * exposure on anxiety-like behavior in the open field test, corticosterone, and hippocampal tyrosine hydroxylase in adolescent and adult mice.
Application : WB
Reactivity : Mouse
Heimovics SA et al. Endocrinology 2012; 153 (3):1364-76 rapid and widespread effects of 17β-* on intracellular signaling in the male songbird brain: a seasonal comparison.
Ellis JM et al. Brain Res 2012; Patterns of phosphorylated tyrosine hydroxylase vary with song production in female starlings.
Application : IHC-Fr
Reactivity : Starling
Matragrano LL et al. PLoS One 2012; 7 (6):e39388 Rapid effects of hearing song on catecholaminergic activity in the songbird auditory pathway.
Application : IHC
Reactivity : Bird
Matragrano LL et al. Eur J Neurosci 2011; 34 (3):416-25 *-dependent catecholaminergic innervation of auditory areas in a seasonally breeding songbird.
Application : IHC
Reactivity : Bird
Gammie SC et al. PLoS One 2008; 3 (4):e1974 Altered * signaling in naturally occurring maternal neglect.
Application : IHC
Reactivity : Mouse
SDS
Glycerol.pdf
Package List Price ($)
$ 479