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Tyrosyl tRNA synthetase antibody

Anti-Tyrosyl tRNA synthetase antibody used in Western Blot (WB). GTX101492
Anti-Tyrosyl tRNA synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101492
Anti-Tyrosyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX101492

Cat. No. GTX101492

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Tyrosyl tRNA synthetase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Tyrosyl tRNA synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952619

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosyl-tRNA synthetase , CMTDIC , TYRRS , YRS , YTS

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Tyrosyl tRNA synthetase antibody used in Western Blot (WB). GTX101492

GTX101492 WB Image

Sample(30 ug whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX101492 diluted at 1:1000

Anti-Tyrosyl tRNA synthetase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101492

GTX101492 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Tyrosyl tRNA synthetase(GTX101492) antibody at 1:200 dilution.

Anti-Tyrosyl tRNA synthetase antibody used in IHC (Paraffin sections) (IHC-P). GTX101492

GTX101492 IHC-P Image

Tyrosyl tRNA synthetase antibody detects YARS protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Tyrosyl tRNA synthetase antibody (GTX101492) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Tsukahara T et al. Peptides 2017; 101 : 60-68 Tyrosyl-tRNA synthetase: A potential kyotorphin synthetase in mammals.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169