GeneTex
United States (US)

Tyrosyl tRNA synthetase antibody

GTX101492_39995_IHC_M_19011506_593.jpg
GTX101492_39995_IFA_19011506_541.jpg
GTX101492_WB_19011506_577.jpg

Cat No. GTX101492

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Tyrosyl tRNA synthetase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosyl-Trna Synthetase,Cmtdic,Tyrrs,Yrs,Yts,Yars

Cellular Localization

Cytoplasm

Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101492_39995_IHC_M_19011506_593.jpg

GTX101492 IHC-P Image

Tyrosyl tRNA synthetase antibody detects YARS protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Tyrosyl tRNA synthetase antibody (GTX101492) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101492_39995_IFA_19011506_541.jpg

GTX101492 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using Tyrosyl tRNA synthetase(GTX101492) antibody at 1:200 dilution.

GTX101492_WB_19011506_577.jpg

GTX101492 WB Image

Sample(30 ug whole cell lysate)
A:H1299
7.5% SDS PAGE
GTX101492 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169