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U1SNRNPBP antibody [N2C3]

Anti-U1SNRNPBP antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119546
Anti-U1SNRNPBP antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX119546
Anti-U1SNRNPBP antibody [N2C3] used in Western Blot (WB). GTX119546
Anti-U1SNRNPBP antibody [N2C3] used in Western Blot (WB). GTX119546

Cat. No. GTX119546

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all U1SNRNPBP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Mouse brain , U87-MG

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human U1SNRNPBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618366

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

small nuclear ribonucleoprotein U11/U12 subunit 35 , HM-1 , U1SNRNPBP

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a homolog of U1-snRNP binding protein. The N-terminal half contains a RNA recognition motif and the C-terminal is rich in Arg/Asp and Arg/Glu dipeptides; a characteristic of a variety of splicing factors. This protein is a component of the U11/U12 small nuclear ribonucleoproteins (snRNP) that form part of the U12-type spliceosome. This gene is differentially expressed in a variety of human tissues. Alternative splicing results in multiple transcript variants encoding distinct proteins.

Database

Research Area

DATA IMAGES

Anti-U1SNRNPBP antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119546

GTX119546 ICC/IF Image

U1SNRNPBP antibody [N2C3] detects U1SNRNPBP protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: U1SNRNPBP protein stained by U1SNRNPBP antibody [N2C3] (GTX119546) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-U1SNRNPBP antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX119546

GTX119546 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using U1SNRNPBP(GTX119546) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-U1SNRNPBP antibody [N2C3] used in Western Blot (WB). GTX119546

GTX119546 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX119546 diluted at 1:1000

Anti-U1SNRNPBP antibody [N2C3] used in Western Blot (WB). GTX119546

GTX119546 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX119546 diluted at 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169