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U2AF1L4 antibody

Anti-U2AF1L4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119283

Cat. No. GTX119283

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all U2AF1L4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human U2AF1L4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885443

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

U2 small nuclear RNA auxiliary factor 1 like 4 , U2AF1-RS3 , U2AF1L3 , U2AF1L3V1 , U2AF1RS3 , U2af26

Cellular Localization

Nucleus , Nucleus speckle

Background

RNA-binding protein that function as a pre-mRNA splicing factor. Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Acts by enhancing the binding of U2AF2 to weak pyrimidine tracts. Also participates in the regulation of alternative pre-mRNA splicing. Activates exon 5 skipping of PTPRC during T cell activation; an event reversed by GFI1. Binds to RNA at the AG dinucleotide at the 3'-splice site.

Database

DATA IMAGES

Anti-U2AF1L4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX119283

GTX119283 ICC/IF Image

U2AF1L4 antibody detects U2AF1L4 protein at nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: U2AF1L4 protein stained by U2AF1L4 antibody (GTX119283) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169