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U2AF35 antibody

Orthogonal
Anti-U2AF35 antibody used in Western Blot (WB). GTX106854
Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX106854
Anti-U2AF35 antibody used in Immunoprecipitation (IP). GTX106854
Anti-U2AF35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106854

Cat. No. GTX106854

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all U2AF35 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952473

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

U2 small nuclear RNA auxiliary factor 1 , FP793 , RN , RNU2AF1 , U2AF35 , U2AFBP

Cellular Localization

Nucleus

Background

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-U2AF35 antibody used in Western Blot (WB). GTX106854

GTX106854 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with U2AF35 antibody (GTX106854) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX106854

GTX106854 IHC-P Image

U2AF35 antibody detects U2AF35 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
U2AF35 antibody (GTX106854) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-U2AF35 antibody used in Immunoprecipitation (IP). GTX106854

GTX106854 IP Image

Immunoprecipitation of U2AF35 protein from 293T whole cell extracts using 5 μg of U2AF35 antibody (GTX106854).
Western blot analysis was performed using U2AF35 antibody (GTX106854).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-U2AF35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106854

GTX106854 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using U2AF35(GTX106854) antibody at 1:200 dilution.

REFERENCE

Application Reference

Mora Gallardo C et al. Nucleic Acids Res 2019; 47 (10) : 5381-5394 Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169