*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
1XPBS (pH7), 20% Glycerol
0.025% ProClin 300
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.34 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
U2 small nuclear RNA auxiliary factor 1 , FP793 , RN , RNU2AF1 , U2AF35 , U2AFBP
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
GTX106854 WB Image
U2AF35 antibody detects U2AF35 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with U2AF35 antibody (GTX106854) diluted by 1:1000.
GTX106854 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using U2AF35(GTX106854) antibody at 1:200 dilution.
GTX106854 IP Image
Immunoprecipitation of U2AF35 protein from 293T whole cell extracts using 5 μg of U2AF35 antibody (GTX106854).
Western blot analysis was performed using U2AF35 antibody (GTX106854).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX106854 IHC-P Image
U2AF35 antibody detects U2AF35 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
U2AF35 antibody (GTX106854) diluted at 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min