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U2AF35 antibody

Anti-U2AF35 antibody used in Western Blot (WB). GTX106854
Anti-U2AF35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106854
Anti-U2AF35 antibody used in Immunoprecipitation (IP). GTX106854
Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX106854

Cat No. GTX106854

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952473

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

U2 small nuclear RNA auxiliary factor 1 , FP793 , RN , RNU2AF1 , U2AF35 , U2AFBP

Cellular Localization

Nucleus

Background

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

DATA IMAGES

Anti-U2AF35 antibody used in Western Blot (WB). GTX106854

GTX106854 WB Image

U2AF35 antibody detects U2AF35 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with U2AF35 antibody (GTX106854) diluted by 1:1000.

Anti-U2AF35 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106854

GTX106854 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using U2AF35(GTX106854) antibody at 1:200 dilution.

Anti-U2AF35 antibody used in Immunoprecipitation (IP). GTX106854

GTX106854 IP Image

Immunoprecipitation of U2AF35 protein from 293T whole cell extracts using 5 μg of U2AF35 antibody (GTX106854).
Western blot analysis was performed using U2AF35 antibody (GTX106854).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX106854

GTX106854 IHC-P Image

U2AF35 antibody detects U2AF35 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
U2AF35 antibody (GTX106854) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169