GeneTex
United States (US)

U2AF35 antibody

GTX106854_IFA_19011506_725.jpg
GTX106854_WB_19011506_841.jpg
GTX106854_39981_20150529_IP_19011506_490.jpg
GTX106854_39981_20141120_IHC_M_19011506_113.jpg

Cat No. GTX106854

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.55mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp313J1712 antibody, FP793 antibody, RN antibody, RNU2AF1 antibody, U2AF35 antibody, U2AFBP antibody, U2AF1 antibody, U2 snRNP auxiliary factor small subunit antibody, U2 small nuclear RNA auxillary factor 1 antibody, "U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit antibody", U2(RNU2) small nuclear RNA auxiliary factor binding protein antibody, splicing factor U2AF 35 kDa subunit antibody, splicing factor U2AF 35kDa subunit antibody, U2 small nuclear RNA auxiliary factor 1 antibody

Cellular Localization

Nucleus

Background

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX106854_IFA_19011506_725.jpg

GTX106854 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using U2AF35(GTX106854) antibody at 1:200 dilution.

GTX106854_WB_19011506_841.jpg

GTX106854 WB Image

Sample(30 µg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
12% SDS PAGE
GTX106854 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106854_39981_20150529_IP_19011506_490.jpg

GTX106854 IP Image

Immunoprecipitation of U2AF35 protein from 293T whole cell extracts using 5 μg of U2AF35 antibody (GTX106854).
Western blot analysis was performed using U2AF35 antibody (GTX106854).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX106854_39981_20141120_IHC_M_19011506_113.jpg

GTX106854 IHC-P Image

U2AF35 antibody detects U2AF35 protein at nucleus on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
U2AF35 antibody (GTX106854) diluted at 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169