APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500 - 1:2000 |
1:50 - 1:100 |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human U2AF1 (NP_006749.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
U2 small nuclear RNA auxiliary factor 1 , FP793 , RN , RNU2AF1 , U2AF35 , U2AFBP
Cellular Localization
Nucleus,Nucleus speckle
Background
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
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GTX55827 WB Image
WB analysis of various sample lysates using GTX55827 U2AF35 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX55827 IHC-P Image
IHC-P analysis of rat intestine tissue using GTX55827 U2AF35 antibody. Dilution : 1:200
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GTX55827 IHC-P Image
IHC-P analysis of human oophoroma tissue using GTX55827 U2AF35 antibody. Dilution : 1:200
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GTX55827 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX55827 U2AF35 antibody. Dilution : 1:200
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REFERENCE
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REVIEW
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