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U2AF35 antibody

Anti-U2AF35 antibody used in Western Blot (WB). GTX55827
Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX55827
Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX55827
Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX55827

Cat No. GTX55827

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human U2AF1 (NP_006749.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

U2 small nuclear RNA auxiliary factor 1 , FP793 , RN , RNU2AF1 , U2AF35 , U2AFBP

Cellular Localization

Nucleus,Nucleus speckle

Background

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-U2AF35 antibody used in Western Blot (WB). GTX55827

GTX55827 WB Image

WB analysis of various sample lysates using GTX55827 U2AF35 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX55827

GTX55827 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX55827 U2AF35 antibody.
Dilution : 1:200

Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX55827

GTX55827 IHC-P Image

IHC-P analysis of human oophoroma tissue using GTX55827 U2AF35 antibody.
Dilution : 1:200

Anti-U2AF35 antibody used in IHC (Paraffin sections) (IHC-P). GTX55827

GTX55827 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX55827 U2AF35 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339