Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with U2AF65 antibody [C1C3] (GTX115622) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using U2AF65(GTX115622) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

U2AF65 antibody [C1C3] detects U2AF65 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: U2AF65 stained by U2AF65 antibody [C1C3] (GTX115622) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

U2AF65 antibody [C1C3] detects U2AF65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
U2AF65 stained by U2AF65 antibody [C1C3] (GTX115622) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

U2AF65 antibody [C1C3] detects U2AF65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
U2AF65 stained by U2AF65 antibody [C1C3] (GTX115622) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min