GeneTex
United States (US)

U2AF65 antibody [C1C3]

GTX115622_40442_WB_18121410_126.jpg
GTX115622_40442_20150324_IFA_18121410_101.jpg
GTX115622_40442_IHC_18121410_877.jpg

Cat No. GTX115622

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human U2AF65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

U2 Small Nuclear Rna Auxiliary Factor 2,U2Af65,U2Af2

Cellular Localization

Nucleus

Background

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]

Database

DATA IMAGES
GTX115622_40442_WB_18121410_126.jpg

GTX115622 WB Image

Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
GTX115622 diluted at 1:10000

GTX115622_40442_20150324_IFA_18121410_101.jpg

GTX115622 ICC/IF Image

U2AF65 antibody [C1C3] detects U2AF65 protein at nucleus by immunofluorescent analysis.
Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: U2AF65 protein stained by U2AF65 antibody [C1C3] (GTX115622) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX115622_40442_IHC_18121410_877.jpg

GTX115622 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using U2AF65(GTX115622) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169