GeneTex

  • Country / Location Selection

United States (US)

U2AF65 antibody [C1C3]

Anti-U2AF65 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115622
Anti-U2AF65 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115622
Anti-U2AF65 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115622
Anti-U2AF65 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115622
Anti-U2AF65 antibody [C1C3] used in Western Blot (WB). GTX115622

Cat. No. GTX115622

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all U2AF65 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Bovine, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human U2AF65. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723099

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

U2 small nuclear RNA auxiliary factor 2 , U2AF65

Cellular Localization

Nucleus

Background

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-U2AF65 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115622

GTX115622 ICC/IF Image

U2AF65 antibody [C1C3] detects U2AF65 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: U2AF65 stained by U2AF65 antibody [C1C3] (GTX115622) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-U2AF65 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115622

GTX115622 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using U2AF65(GTX115622) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-U2AF65 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115622

GTX115622 IHC-P Image

U2AF65 antibody [C1C3] detects U2AF65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
U2AF65 stained by U2AF65 antibody [C1C3] (GTX115622) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-U2AF65 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115622

GTX115622 IHC-P Image

U2AF65 antibody [C1C3] detects U2AF65 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
U2AF65 stained by U2AF65 antibody [C1C3] (GTX115622) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-U2AF65 antibody [C1C3] used in Western Blot (WB). GTX115622

GTX115622 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with U2AF65 antibody [C1C3] (GTX115622) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for U2AF65 antibody [C1C3] (GTX115622). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169