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UAP1 antibody [N1C3]

Anti-UAP1 antibody [N1C3] used in Western Blot (WB). GTX103592
Anti-UAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103592
Anti-UAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103592
Anti-UAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103592

Cat. No. GTX103592

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952476

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UDP-N-acetylglucosamine pyrophosphorylase 1 , AGX , AGX1 , AGX2 , SPAG2

Cellular Localization

Cytoplasm

Background

Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.

Database

Research Area

DATA IMAGES

Anti-UAP1 antibody [N1C3] used in Western Blot (WB). GTX103592

GTX103592 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX103592 diluted at 1:3000

Anti-UAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103592

GTX103592 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using UAP1(GTX103592) antibody at 1:200 dilution.

Anti-UAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103592

GTX103592 ICC/IF Image

UAP1 antibody [N1C3] detects UAP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UAP1 protein stained by UAP1 antibody [N1C3] (GTX103592) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-UAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103592

GTX103592 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES-2 xenograft , using UAP1(GTX103592) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Andres LM et al. ACS Chem Biol 2017; 12 (8) : 2030-2039 Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation.
Yu SH et al. Proc Natl Acad Sci U S A 2012; 109 (13) : 1071867 Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169