GeneTex
United States (US)

UAP1 antibody [N1C3]

GTX103592_IHC_19011506_902.jpg
GTX103592_WB_19011506_591.jpg
GTX103592_39995_20150410_IFA_19011506_929.jpg
GTX103592_IFA_19011506_826.jpg

Cat No. GTX103592

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AGX antibody, AGX1 antibody, AGX2 antibody, SPAG2 antibody, UAP1 antibody, sperm-associated antigen 2 antibody, sperm associated antigen 2 antibody, antigen X antibody, UDP-N-acetylhexosamine pyrophosphorylase antibody, UDP-N-acteylglucosamine pyrophosphorylase 1 antibody

Cellular Localization

Cytoplasm

Background

Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.

Database

DATA IMAGES
GTX103592_IHC_19011506_902.jpg

GTX103592 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES-2 xenograft , using UAP1(GTX103592) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX103592_WB_19011506_591.jpg

GTX103592 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX103592 diluted at 1:3000

GTX103592_39995_20150410_IFA_19011506_929.jpg

GTX103592 ICC/IF Image

UAP1 antibody [N1C3] detects UAP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UAP1 protein stained by UAP1 antibody [N1C3] (GTX103592) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX103592_IFA_19011506_826.jpg

GTX103592 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using UAP1(GTX103592) antibody at 1:200 dilution.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169