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UAP1 antibody [N1C3]

Anti-UAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103592
Anti-UAP1 antibody [N1C3] used in Western Blot (WB). GTX103592
Anti-UAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103592
Anti-UAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103592

Cat No. GTX103592

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

UDP-N-acetylglucosamine pyrophosphorylase 1 , AGX , AGX1 , AGX2 , SPAG2

Cellular Localization

Cytoplasm

Background

Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.

Database

DATA IMAGES
Anti-UAP1 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103592

GTX103592 IHC-P Image

Immunohistochemical analysis of paraffin-embedded ES-2 xenograft , using UAP1(GTX103592) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UAP1 antibody [N1C3] used in Western Blot (WB). GTX103592

GTX103592 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX103592 diluted at 1:3000

Anti-UAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103592

GTX103592 ICC/IF Image

UAP1 antibody [N1C3] detects UAP1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UAP1 protein stained by UAP1 antibody [N1C3] (GTX103592) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-UAP1 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103592

GTX103592 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using UAP1(GTX103592) antibody at 1:200 dilution.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169