GeneTex
United States (US)

UBA2 antibody [N1N3]

GTX102613_WB_19011506_294.jpg
GTX102613_40191_IFA_19011506_585.jpg

Cat No. GTX102613

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human UBA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ARX antibody, FLJ13058 antibody, SAE2 antibody, UBA2 antibody, ubiquitin-like 1-activating enzyme E1B antibody, SUMO1 activating enzyme subunit 2 antibody, anthracycline-associated resistance ARX antibody, "UBA2, ubiquitin-activating enzyme E1 homolog antibody", SUMO-1 activating enzyme subunit 2 antibody, SUMO-activating enzyme subunit 2 antibody, ubiquitin-like modifier activating enzyme 2 antibody

Cellular Localization

Nucleus

Background

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM]

Database

DATA IMAGES
GTX102613_WB_19011506_294.jpg

GTX102613 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
UBA2 antibody
GTX102613 diluted at 1:1000

GTX102613_40191_IFA_19011506_585.jpg

GTX102613 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBA2(GTX102613) antibody at 1:500 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169