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UBA2 antibody

Anti-UBA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31804
Anti-UBA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31804
Anti-UBA2 antibody used in Western Blot (WB). GTX31804

Cat. No. GTX31804

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all UBA2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25 μg/mL
ICC/IF 4 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

UBA2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human UBA2.The immunogen is located within amino acids 550 - 600 of UBA2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin like modifier activating enzyme 2 , ARX , HRIHFB2115 , SAE2

Cellular Localization

Cytoplasm,Nucleus

Background

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-UBA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31804

GTX31804 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31804 UBA2 antibody.
Working concentration : 4 μg/ml

Anti-UBA2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31804

GTX31804 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31804 UBA2 antibody.
Working concentration : 20 μg/ml

Anti-UBA2 antibody used in Western Blot (WB). GTX31804

GTX31804 WB Image

WB analysis of 293 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX31804 UBA2 antibody.
Working concentration : 0.25 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399