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UBASH3A antibody [N1C1]

Anti-UBASH3A antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116432
Anti-UBASH3A antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116432
Anti-UBASH3A antibody [N1C1] used in Western Blot (WB). GTX116432

Cat. No. GTX116432

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UBASH3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

Positive Control

K562

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBASH3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624277

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin associated and SH3 domain containing A , CLIP4 , STS-2 , TULA , TULA-1

Cellular Localization

Cytoplasm , Nucleus

Database

Research Area

DATA IMAGES

Anti-UBASH3A antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX116432

GTX116432 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using UBASH3A(GTX116432) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UBASH3A antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116432

GTX116432 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBASH3A(GTX116432) antibody at 1:500 dilution.

Anti-UBASH3A antibody [N1C1] used in Western Blot (WB). GTX116432

GTX116432 WB Image

Sample (30 ug of whole cell lysate)
A: K562
10% SDS PAGE
GTX116432 diluted at 1:2000

REFERENCE

Application Reference

Yamagata K et al. Inflamm Regen 2022; 42 (1) : 46 IL-6 production through repression of UBASH3A gene via epigenetic dysregulation of super-enhancer in CD4(+) T cells in rheumatoid arthritis.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169