GeneTex
United States (US)

UBE1 antibody [N1N3]

GTX114866_40632_WB_18121410_339.jpg

Cat No. GTX114866

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IP Assay dependent
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , MCF-7

Predict Reactivity

Mouse, Rat, Rabbit, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Like Modifier Activating Enzyme 1,A1S9,A1S9T,A1St,Amcx1,Cfap124,Gxp1,Poc20,Smax2,Uba1A,Ube1,Ube1X,Uba1

Background

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114866_40632_WB_18121410_339.jpg

GTX114866 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
B: U87-MG
7.5% SDS PAGE
GTX114866 diluted at 1:10000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169