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UBE1 antibody

Anti-UBE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66102
Anti-UBE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66102
Anti-UBE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66102
Anti-UBE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66102
Anti-UBE1 antibody used in Western Blot (WB). GTX66102

Cat. No. GTX66102

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all UBE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

118 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_695012.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin like modifier activating enzyme 1 , A1S9 , A1S9T , A1ST , AMCX1 , CFAP124 , GXP1 , POC20 , SMAX2 , UBA1A , UBE1 , UBE1X

Cellular Localization

Cytoplasm,Mitochondrion,Nucleus

Background

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-UBE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66102

GTX66102 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX66102 UBE1 antibody.
Dilution : 1:100

Anti-UBE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66102

GTX66102 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX66102 UBE1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-UBE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66102

GTX66102 IHC-P Image

IHC-P analysis of rat liver tissue using GTX66102 UBE1 antibody.
Dilution : 1:100

Anti-UBE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX66102

GTX66102 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX66102 UBE1 antibody.
Dilution : 1:100

Anti-UBE1 antibody used in Western Blot (WB). GTX66102

GTX66102 WB Image

WB analysis of various sample lysates using GTX66102 UBE1 antibody.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399