GeneTex
United States (US)

UBE1L antibody [N1N2], N-term

GTX104103_WB_19011506_454.jpg
GTX104103_40002_IHC_19011506_578.jpg

Cat No. GTX104103

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

112 kDa. ( Note )

Positive Control

A431 , HeLaS3 , HepG2 , Molt-4

Predict Reactivity

Bovine, Cat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human UBE1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Like Modifier Activating Enzyme 7,D8,Uba1B,Ube1L,Ube2,Uba7

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104103_WB_19011506_454.jpg

GTX104103 WB Image

Sample(30 ug whole cell lysate)
A:A431(GTX27909)
7.5% SDS PAGE
GTX104103 diluted at 1:1000

GTX104103_40002_IHC_19011506_578.jpg

GTX104103 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using UBE1L(GTX104103) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf