Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UBE1L antibody [N1N2], N-term (GTX104103) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using UBE1L(GTX104103) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min