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UBE2A antibody

Anti-UBE2A antibody used in Western Blot (WB). GTX100426
Anti-UBE2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100426

Cat No. GTX100426

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

HeLa

Specificity/Sensitivity

Specificity: Based on sequence homology, this antibody may cross react with UBE2B protein.

Predict Reactivity

Mouse, Rat, Zebrafish, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human UBE2A Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 A , HHR6A , MRXS30 , MRXSN , RAD6A , UBC2

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UBE2A antibody used in Western Blot (WB). GTX100426

GTX100426 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX100426 diluted at 1:1000

Anti-UBE2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100426

GTX100426 ICC/IF Image

UBE2A antibody detects UBE2A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBE2A protein stained by UBE2A antibody (GTX100426) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf