*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Specificity: Based on sequence homology, this antibody may cross react with UBE2B protein.
Mouse, Rat, Zebrafish, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
1XPBS (pH7), 20% Glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.93 mg/ml (Please refer to the vial label for the specific concentration.)
Full length human UBE2A Recombinant protein.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ubiquitin conjugating enzyme E2 A , HHR6A , MRXS30 , MRXSN , RAD6A , UBC2
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq]
GTX100426 ICC/IF Image
UBE2A antibody detects UBE2A protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBE2A protein stained by UBE2A antibody (GTX100426) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX100426 WB Image
UBE2A antibody detects UBE2A protein by Western blot analysis.
A. 30 μg K562 whole cell lysate/extract
B. 30 μg THP-1 whole cell lysate/extract
C. 30 μg HL-60 whole cell lysate/extract
15 % SDS-PAGE
UBE2A antibody (GTX100426) dilution: 1:1000